Cytological Changes in the Oral Mucosa After Use of a Mouth Rinse with Alcohol. A Prospective Double Blind Control Study

Overview

Journal Med Oral Patol Oral Cir Bucal

Specialty Dentistry

Date 2012 Oct 23

PMID 23085712

Citations 6

Authors

Jose V Bagan

Francisco Vera-Sempere

Cristina Marzal

Ana Pellin-Carcelen

Ezequiel Marti-Bonmati

Leticia Bagan

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this preliminary study was to detect cytological changes in the oral mucosa after using a mouth wash with alcohol.

Material And Methods: A prospective double-blind, controlled study was performed, for 6 months. Group 1 consisted of 30 subjects who used a mouth rinse with 26.9% of alcohol [Listerine] and Group 2 consisted of 30 subjects who used a mouth rinse with the same ingredients but with no alcohol. We obtained three cytological samples from the oral mucosa. The presence of cytological atypia, binucleation and karyorrhesis, and type of cells were studied. We also used a fluorescent in situ hybridization technique (FISH) in 15 samples in each group, for the micronucleus.

Results: We found no clinical mucosal alteration after using the mouth wash at the end of the study in either group. We observed no cytological differences between the groups at the end of the study (p>0.05). Regarding the study of the micronucleus by FISH, we observed no significant difference between the groups (p>0.05).

Conclusions: Our results showed no cytological alteration in patients using a mouth rinse with alcohol, but these findings should be considered preliminary results, to be confirmed in a greater sample of patients.

Citing Articles

Preprocedural mouthwashes for infection control in dentistry-an update.

Weber J, Bonn E, Auer D, Kirschneck C, Buchalla W, Scholz K Clin Oral Investig. 2023; 27(Suppl 1):33-44.

PMID: 37079156 PMC: 10116478. DOI: 10.1007/s00784-023-04953-z.

Evaluation of the effect of Jaftex herbal mouthwash on the growth of and .

Amin M, Babadi F, Baghipour N, Sadeghi-Nejad B J Family Med Prim Care. 2021; 10(10):3815-3819.

PMID: 34934685 PMC: 8653495. DOI: 10.4103/jfmpc.jfmpc_1067_21.

Efficacy and safety evaluation of alcohol-containing and alcohol-free mouth rinses: A clinicocytological study.

Gupta V, Pant V, Pandey S, Pant A J Indian Soc Periodontol. 2021; 25(2):128-132.

PMID: 33888944 PMC: 8041073. DOI: 10.4103/jisp.jisp_196_20.

Adverse events associated with home use of mouthrinses: a systematic review.

Tartaglia G, Tadakamadla S, Connelly S, Sforza C, Martin C Ther Adv Drug Saf. 2019; 10:2042098619854881.

PMID: 31579502 PMC: 6759706. DOI: 10.1177/2042098619854881.

Antibacterial Activity of Essential Oils with and without Alcohol on Oral Biofilm: A Randomized Clinical Trial.

Quintas V, Prada-Lopez I, Carreira M, Suarez-Quintanilla D, Balsa-Castro C, Tomas I Front Microbiol. 2017; 8:2162.

PMID: 29218030 PMC: 5703870. DOI: 10.3389/fmicb.2017.02162.

References

Natarajan A . Fluorescence in situ hybridization (FISH) in genetic toxicology. J Environ Pathol Toxicol Oncol. 2002; 20(4):293-8. View

Loe H . The Gingival Index, the Plaque Index and the Retention Index Systems. J Periodontol. 1967; 38(6):Suppl:610-6. DOI: 10.1902/jop.1967.38.6.610. View

Patel M, Ndlovu N, Owen C, Veale R . Properties of a new mouthrinse for patients receiving radiation therapy. SADJ. 2010; 65(9):410, 412-4. View

Besaratinia A, Pfeifer G . DNA-lesion mapping in mammalian cells. Methods. 2009; 48(1):35-9. PMC: 2693248. DOI: 10.1016/j.ymeth.2009.02.008. View

Koschier F, Kostrubsky V, Toole C, Gallo M . In vitro effects of ethanol and mouthrinse on permeability in an oral buccal mucosal tissue construct. Food Chem Toxicol. 2011; 49(10):2524-9. DOI: 10.1016/j.fct.2011.06.018. View

Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E . Buccal micronucleus cytome assay. Nat Protoc. 2009; 4(6):825-37. DOI: 10.1038/nprot.2009.53. View

Chen Y, Jin C, Zhang X, Ge S, Zhang Z, Xu H . Seventeen-year follow-up study on chromosomal aberrations in five victims accidentally exposed to several Gy of 60Co gamma-rays. Radiat Environ Biophys. 2008; 48(1):57-65. DOI: 10.1007/s00411-008-0203-y. View

Sarto F, Finotto S, Giacomelli L, Mazzotti D, Tomanin R, LEVIS A . The micronucleus assay in exfoliated cells of the human buccal mucosa. Mutagenesis. 1987; 2(1):11-7. DOI: 10.1093/mutage/2.1.11. View

Tolbert P, Shy C, Allen J . Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res. 1992; 271(1):69-77. DOI: 10.1016/0165-1161(92)90033-i. View

10.

Svatun B, Heloe L . Dental status and treatment needs among institutionalized mentally subnormal persons in Norway. Community Dent Oral Epidemiol. 1975; 3(5):208-13. DOI: 10.1111/j.1600-0528.1975.tb00310.x. View

11.

Guha N, Boffetta P, Wunsch Filho V, Neto J, Shangina O, Zaridze D . Oral health and risk of squamous cell carcinoma of the head and neck and esophagus: results of two multicentric case-control studies. Am J Epidemiol. 2007; 166(10):1159-73. DOI: 10.1093/aje/kwm193. View

12.

Moghadam B, Gier R, Thurlow T . Extensive oral mucosal ulcerations caused by misuse of a commercial mouthwash. Cutis. 1999; 64(2):131-4. View

13.

Turkez H, Togar B, Arabaci T . Evaluation of genotoxicity after application of Listerine(R) on human lymphocytes by micronucleus and single cell gel electrophoresis assays. Toxicol Ind Health. 2011; 28(3):271-5. DOI: 10.1177/0748233711410918. View

14.

Gordon J, Lamster I, Seiger M . Efficacy of Listerine antiseptic in inhibiting the development of plaque and gingivitis. J Clin Periodontol. 1985; 12(8):697-704. DOI: 10.1111/j.1600-051x.1985.tb00941.x. View

15.

Amini P, Araujo M, Wu M, Charles C, Sharma N . Comparative antiplaque and antigingivitis efficacy of three antiseptic mouthrinses: a two week randomized clinical trial. Braz Oral Res. 2009; 23(3):319-25. DOI: 10.1590/s1806-83242009000300016. View

16.

Bonassi S, Biasotti B, Kirsch-Volders M, Knasmueller S, Zeiger E, Burgaz S . State of the art survey of the buccal micronucleus assay--a first stage in the HUMN(XL) project initiative. Mutagenesis. 2009; 24(4):295-302. DOI: 10.1093/mutage/gep019. View

17.

McCullough M, Farah C . The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes. Aust Dent J. 2009; 53(4):302-5. DOI: 10.1111/j.1834-7819.2008.00070.x. View

18.

Elmore J, Horwitz R . Oral cancer and mouthwash use: evaluation of the epidemiologic evidence. Otolaryngol Head Neck Surg. 1995; 113(3):253-61. DOI: 10.1016/S0194-5998(95)70114-1. View

19.

Garcia-Sagredo J . Fifty years of cytogenetics: a parallel view of the evolution of cytogenetics and genotoxicology. Biochim Biophys Acta. 2008; 1779(6-7):363-75. DOI: 10.1016/j.bbagrm.2008.05.003. View

20.

Cole P, Rodu B, Mathisen A . Alcohol-containing mouthwash and oropharyngeal cancer: a review of the epidemiology. J Am Dent Assoc. 2003; 134(8):1079-87. DOI: 10.14219/jada.archive.2003.0322. View