Inhibition of Akt Attenuates RPO-Induced Cardioprotection

Overview

Journal Cardiol Res Pract

Publisher Wiley

Specialty Cardiology & Vascular Diseases

Date 2012 Oct 20

PMID 23082275

Citations 3

Authors

Emma Katengua-Thamahane

Anna-Mart Engelbrecht

Adriaan J Esterhuyse

Jacques van Rooyen

Affiliations

Soon will be listed here.

Abstract

Previous studies have shown that red palm oil (RPO) supplementation protected rat hearts against ischaemia-reperfusion injury. Evidence from these studies suggested that Akt may be partly responsible for the observed protection. The aim of the current study was therefore to prove or refute the involvement of Akt in the RPO-induced cardioprotection by administration of a specific Akt inhibitor (A6730). Male Wistar rats were randomly divided into 2 groups: a control group receiving standard rat chow and an experimental group receiving standard rat chow plus 2 mL RPO for six weeks. Hearts were excised and mounted on the Langendorff perfusion system. Functional recovery was documented. A different set of hearts were freeze-clamped to assess total and phosphorylation status of Akt. Another set of hearts were subjected to the same perfusion conditions with addition of A6730. Hearts from this protocol were freeze-clamped and assessed for total and phospho-Akt. RPO improved functional recovery which was associated with increased phosphorylation of Akt on Ser473 and Thr308 residues. Blockade of Akt phosphorylation caused poor functional recovery. For the first time, these results prove that Akt plays an important role in the RPO-induced cardioprotection.

Citing Articles

Effects of Red Palm Oil on Myocardial Antioxidant Enzymes, Nitric Oxide Synthase and Heart Function in Spontaneously Hypertensive Rats.

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PMID: 29160855 PMC: 5713442. DOI: 10.3390/ijms18112476.

Berbamine postconditioning protects the heart from ischemia/reperfusion injury through modulation of autophagy.

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Modulation of Hypercholesterolemia-Induced Oxidative/Nitrative Stress in the Heart.

Csonka C, Sarkozy M, Pipicz M, Dux L, Csont T Oxid Med Cell Longev. 2016; 2016:3863726.

PMID: 26788247 PMC: 4691632. DOI: 10.1155/2016/3863726.

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