Prion Remains Infectious After Passage Through Digestive System of American Crows (Corvus Brachyrhynchos)

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Oct 20

PMID 23082115

Citations 15

Authors

Kurt C VerCauteren

John L Pilon

Paul B Nash

Gregory E Phillips

Justin W Fischer

Affiliations

Soon will be listed here.

Abstract

Avian scavengers, such as American crows (Corvus brachyrhynchos), have potential to translocate infectious agents (prions) of transmissible spongiform encephalopathy (TSE) diseases including chronic wasting disease, scrapie, and bovine spongiform encephalopathy. We inoculated mice with fecal extracts obtained from 20 American crows that were force-fed material infected with RML-strain scrapie prions. These mice all evinced severe neurological dysfunction 196-231 d postinoculation (x =198; 95% CI: 210-216) and tested positive for prion disease. Our results suggest a large proportion of crows that consume prion-positive tissue are capable of passing infectious prions in their feces (ˆp=1.0; 95% CI: 0.8-1.0). Therefore, this common, migratory North American scavenger could play a role in the geographic spread of TSE diseases.

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References

Martinsen T, Taylor D, Johnsen R, Waldum H . Gastric acidity protects mice against prion infection?. Scand J Gastroenterol. 2002; 37(5):497-500. DOI: 10.1080/00365520252903017. View

Appel T, Lucassen R, Groschup M, Joncic M, Beekes M, Riesner D . Acid inactivation of prions: efficient at elevated temperature or high acid concentration. J Gen Virol. 2006; 87(Pt 5):1385-1394. DOI: 10.1099/vir.0.81426-0. View

Kruger D, Thomzig A, Lenz G, Kampf K, McBride P, Beekes M . Faecal shedding, alimentary clearance and intestinal spread of prions in hamsters fed with scrapie. Vet Res. 2008; 40(1):4. PMC: 2695018. DOI: 10.1051/vetres:2008042. View

Kascsak R, Rubenstein R, Merz P, Carp R, Wisniewski H, Diringer H . Biochemical differences among scrapie-associated fibrils support the biological diversity of scrapie agents. J Gen Virol. 1985; 66 ( Pt 8):1715-22. DOI: 10.1099/0022-1317-66-8-1715. View

Bessen R, Marsh R . Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent. J Virol. 1992; 66(4):2096-101. PMC: 289000. DOI: 10.1128/JVI.66.4.2096-2101.1992. View

Nicholson E, Richt J, Rasmussen M, Hamir A, Lebepe-Mazur S, Horst R . Exposure of sheep scrapie brain homogenate to rumen-simulating conditions does not result in a reduction of PrP(Sc) levels. Lett Appl Microbiol. 2007; 44(6):631-6. DOI: 10.1111/j.1472-765X.2007.02124.x. View

Miller M, Williams E, Hobbs N, Wolfe L . Environmental sources of prion transmission in mule deer. Emerg Infect Dis. 2004; 10(6):1003-6. PMC: 3323154. DOI: 10.3201/eid1006.040010. View

Wisniewski H, Sigurdarson S, Rubenstein R, Kascsak R, Carp R . Mites as vectors for scrapie. Lancet. 1996; 347(9008):1114. DOI: 10.1016/s0140-6736(96)90310-4. View

Jeffrey M, Gonzalez L, Espenes A, McL Press C, Martin S, Chaplin M . Transportation of prion protein across the intestinal mucosa of scrapie-susceptible and scrapie-resistant sheep. J Pathol. 2006; 209(1):4-14. DOI: 10.1002/path.1962. View

10.

Miller M, Hobbs N, Tavener S . Dynamics of prion disease transmission in mule deer. Ecol Appl. 2007; 16(6):2208-14. DOI: 10.1890/1051-0761(2006)016[2208:dopdti]2.0.co;2. View

11.

Prusiner S . Novel proteinaceous infectious particles cause scrapie. Science. 1982; 216(4542):136-44. DOI: 10.1126/science.6801762. View

12.

HUNTER G, MILLSON G . Attempts to release the scrapie agent from tissue debris. J Comp Pathol. 1967; 77(3):301-7. DOI: 10.1016/0021-9975(67)90039-4. View

13.

CHANDLER R . Encephalopathy in mice produced by inoculation with scrapie brain material. Lancet. 1961; 1(7191):1378-9. DOI: 10.1016/s0140-6736(61)92008-6. View

14.

Peretz D, Supattapone S, Giles K, Vergara J, Freyman Y, Lessard P . Inactivation of prions by acidic sodium dodecyl sulfate. J Virol. 2005; 80(1):322-31. PMC: 1317507. DOI: 10.1128/JVI.80.1.322-331.2006. View

15.

Langeveld J, Wang J, van de Wiel D, Shih G, Garssen G, Bossers A . Enzymatic degradation of prion protein in brain stem from infected cattle and sheep. J Infect Dis. 2003; 188(11):1782-9. DOI: 10.1086/379664. View

16.

Scherbel C, Pichner R, Groschup M, Mueller-Hellwig S, Scherer S, Dietrich R . Degradation of scrapie associated prion protein (PrPSc) by the gastrointestinal microbiota of cattle. Vet Res. 2006; 37(5):695-703. DOI: 10.1051/vetres:2006024. View

17.

Dagleish M, Hamilton S, Gonzalez L, Eaton S, Steele P, Finlayson J . Digestion and transportation of bovine spongiform encephalopathy-derived prion protein in the sheep intestine. J Gen Virol. 2010; 91(Pt 12):3116-23. DOI: 10.1099/vir.0.025049-0. View

18.

Matthews D, Cooke B . The potential for transmissible spongiform encephalopathies in non-ruminant livestock and fish. Rev Sci Tech. 2003; 22(1):283-96. DOI: 10.20506/rst.22.1.1393. View

19.

Kuczius T, Groschup M . Differences in proteinase K resistance and neuronal deposition of abnormal prion proteins characterize bovine spongiform encephalopathy (BSE) and scrapie strains. Mol Med. 1999; 5(6):406-18. PMC: 2230428. View

20.

Seidel B, Thomzig A, Buschmann A, Groschup M, Peters R, Beekes M . Scrapie Agent (Strain 263K) can transmit disease via the oral route after persistence in soil over years. PLoS One. 2007; 2(5):e435. PMC: 1855989. DOI: 10.1371/journal.pone.0000435. View