Enhancing Genotyping of MAOA-LPR and 5-HTT-LPR in Rhesus Macaques (Macaca Mulatta)

Overview

Journal J Med Primatol

Publisher Wiley

Date 2012 Oct 20

PMID 23078595

Citations 9

Authors

Genesio M Karere

Erin Sullivan

Erin L Kinnally

John P Capitanio

Leslie A Lyons

Affiliations

Soon will be listed here.

Abstract

Background: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior.

Methods: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data.

Results: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective.

Conclusions: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

Citing Articles

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Variation in the serotonin transporter genotype is associated with maternal restraint and rejection of infants: A nonhuman primate (Macaca mulatta) model.

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