Identification and Characterization of Plasmids from the Western Aster Yellows Mycoplasmalike Organism

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 Mar 1

PMID 2307660

Citations 3

Authors

C R Kuske

B C Kirkpatrick

Affiliations

Soon will be listed here.

Abstract

Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO) as the probe, identified at least four plasmids in celery, aster, and periwinkle plants and in Macrosteles severini leafhopper vectors infected with either the dwarf AY-MLO, Tulelake AY-MLO, or SAY-MLO strain. Plasmids were also detected in two California field isolates of AY-MLO but not in plants infected with the beet leafhopper-transmitted virescence agent, western X, or elm yellows MLOs. SAY-MLO plasmids were 5.2, 4.9, 3.4, and 1.7 kilobase pairs in size. Plasmids isolated from dwarf AY- and Tulelake AY-MLOs were 7.4, 5.1, 3.5, and 1.7 kilobase pairs in size. No evidence was obtained for integration of SAY-MLO plasmids into the MLO chromosome.

Citing Articles

Cloning and detection of chromosomal and extrachromosomal DNA from mycoplasmalike organisms that cause yellow dwarf disease of rice.

Nakashima K, Kato S, Iwanami S, Murata N Appl Environ Microbiol. 1991; 57(12):3570-5.

PMID: 16348605 PMC: 184014. DOI: 10.1128/aem.57.12.3570-3575.1991.

Mycoplasmalike organisms (MLOs) and distinguish them from other MLOs.

Nakashima K, Kato S, Iwanami S, Murata N Appl Environ Microbiol. 1993; 59(4):1206-12.

PMID: 8489230 PMC: 202262. DOI: 10.1128/aem.59.4.1206-1212.1993.

Mycoplasma-like organisms and ophthalmic disease.

WIROSTKO E, Johnson L, Wirostko B, Farris R Trans Am Ophthalmol Soc. 1993; 91:85-94; discussion 95-8.

PMID: 8140710 PMC: 1298461.

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