A Novel Insight into the Oxidoreductase Activity of Helicobacter Pylori HP0231 Protein

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Oct 12

PMID 23056345

Citations 22

Authors

Paula Roszczenko

Katarzyna A Radomska

Ewa Wywial

Jean-Francois Collet

Elzbieta K Jagusztyn-Krynicka

Affiliations

Soon will be listed here.

Abstract

Background: The formation of a disulfide bond between two cysteine residues stabilizes protein structure. Although we now have a good understanding of the Escherichia coli disulfide formation system, the machineries at work in other bacteria, including pathogens, are poorly characterized. Thus, the objective of this work was to improve our understanding of the disulfide formation machinery of Helicobacter pylori, a leading cause of ulcers and a risk factor for stomach cancer worldwide.

Methods And Results: The protein HP0231 from H. pylori, a structural counterpart of E. coli DsbG, is the focus of this research. Its function was clarified by using a combination of biochemical, microbiological and genetic approaches. In particular, we determined the biochemical properties of HP0231 as well as its redox state in H. pylori cells.

Conclusion: Altogether our results show that HP0231 is an oxidoreductase that catalyzes disulfide bond formation in the periplasm. We propose to call it HpDsbA.

Citing Articles

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Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of and Their Impact on Bacterial Physiology and Pathogenesis.

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Roszczenko-Jasinska P, Wojtys M, Jagusztyn-Krynicka E Appl Microbiol Biotechnol. 2020; 104(23):9891-9905.

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