Precise Mapping of the CD95 Pre-ligand Assembly Domain

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Oct 11

PMID 23049989

Citations 8

Authors

Valerie Edmond

Benoist Ghali

Aubin Penna

Jean-Luc Taupin

Sophie Daburon

Jean-Francois Moreau

Patrick Legembre

Affiliations

Soon will be listed here.

Abstract

Pre-association of CD95 at the plasma membrane is mandatory for efficient death receptor signaling. This homotrimerization occurs through self-association of an extracellular domain called the pre-ligand assembly domain (PLAD). Using novel molecular and cellular tools, we confirmed that CD95-PLAD is necessary to promote CD95 multimerization and plays a pivotal role in the transmission of apoptotic signals. However, while a human CD95 mutant deleted of the previously described PLAD domain (amino acids 1 to 66) fails to interact with its wild-type counterpart and trigger autonomous cell death, deletion of amino acids 1 to 42 does not prevent homo- or hetero (human/mouse)-oligomerization of CD95, and thus does not alter transmission of the apoptotic signal. Overall, these findings indicate that the region between amino acids 43 to 66 corresponds to the minimal motif involved in CD95 homotypic interaction and is necessary to convey an efficient apoptotic signal. Interfering with this PLAD may represent a new therapeutic strategy for altering CD95-induced apoptotic and non-apoptotic signals.

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