Maize Chloroplast RNA Polymerase: the 180-, 120-, and 38-kilodalton Polypeptides Are Encoded in Chloroplast Genes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Feb 1

PMID 2304916

Citations 54

Authors

J Hu

L Bogorad

Affiliations

Soon will be listed here.

Abstract

Prominent polypeptides with apparent molecular masses of 180, 120, 85, and 38 kDa are found in an extensively purified preparation of maize chloroplast DNA-dependent RNA polymerase that retains the capacity to initiate transcription of the cloned chloroplast gene rbcL correctly and the requirement for a supercoiled DNA template for specific and active transcription. Amino-terminal amino acid sequences of the 180-, 120-, and 38-kDa polypeptides have been determined and found to correspond precisely to the sequences deduced from the 5' ends of the maize chloroplast rpoC2, rpoB, and rpoA genes, respectively. These experiments show that these chloroplast rpo genes encode the prominent polypeptides in the highly enriched maize chloroplast RNA polymerase preparation and support the conclusion that these polypeptides are functional components of the enzyme. The rpoB, rpoC1, and rpoC2 genes have been mapped on the maize chloroplast chromosome.

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References

Audren H, Bisanz-Seyer C, Briat J, Mache R . Structure and transcription of the 5S rRNA gene from spinach chloroplasts. Curr Genet. 1987; 12(4):263-9. DOI: 10.1007/BF00435288. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hallick R, Alt J, Westhoff P, Herrmann R . Spinach plastid genes coding for initiation factor IF-1, ribosomal protein S11 and RNA polymerase alpha-subunit. Nucleic Acids Res. 1986; 14(2):1029-44. PMC: 339481. DOI: 10.1093/nar/14.2.1029. View

Meinke A, Igloi G, Kossel H . Nucleotide sequence of tDNA(Cys)GCA and its flanking regions from Zea mays chloroplasts. Nucleic Acids Res. 1988; 16(12):5696. PMC: 336803. DOI: 10.1093/nar/16.12.5696. View

Kidd G, Bogorad L . A facile procedure for purifying maize chloroplast RNA polymerase from whole cell homogenates. Biochim Biophys Acta. 1980; 609(1):14-30. DOI: 10.1016/0005-2787(80)90197-5. View

Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M . The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet. 1989; 217(2-3):185-94. DOI: 10.1007/BF02464880. View

Zaitlin D, Hu J, Bogorad L . Binding and transcription of relaxed DNA templates by fractions of maize chloroplast extracts. Proc Natl Acad Sci U S A. 1989; 86(3):876-80. PMC: 286581. DOI: 10.1073/pnas.86.3.876. View

Russell D, Bogorad L . Transcription analysis of the maize chloroplast gene for the ribosomal protein S4. Nucleic Acids Res. 1987; 15(4):1853-67. PMC: 340586. DOI: 10.1093/nar/15.4.1853. View

Smith H, Bogorad L . The polypeptide subunit structure of the DNA-dependent RNA polymerase of Zea mays chloroplasts. Proc Natl Acad Sci U S A. 1974; 71(12):4839-42. PMC: 433993. DOI: 10.1073/pnas.71.12.4839. View

10.

Purton S, Gray J . The plastid rpoA gene encoding a protein homologous to the bacterial RNA polymerase alpha subunit is expressed in pea chloroplasts. Mol Gen Genet. 1989; 217(1):77-84. DOI: 10.1007/BF00330945. View

11.

Crossland L, Rodermel S, Bogorad L . Single gene for the large subunit of ribulosebisphosphate carboxylase in maize yields two differentially regulated mRNAs. Proc Natl Acad Sci U S A. 1984; 81(13):4060-4. PMC: 345368. DOI: 10.1073/pnas.81.13.4060. View

12.

Little M, Hallick R . Chloroplast rpoA, rpoB, and rpoC genes specify at least three components of a chloroplast DNA-dependent RNA polymerase active in tRNA and mRNA transcription. J Biol Chem. 1988; 263(28):14302-7. View

13.

Jolly S, Bogorad L . Preferential transcription of cloned maize chloroplast DNA sequences by maize chloroplast RNA polymerase. Proc Natl Acad Sci U S A. 1980; 77(2):822-6. PMC: 348373. DOI: 10.1073/pnas.77.2.822. View

14.

Lerbs S, Brautigam E, Parthier B . Polypeptides of DNA-dependent RNA polymerase of spinach chloroplasts: characterization by antibody-linked polymerase assay and determination of sites of synthesis. EMBO J. 1985; 4(7):1661-6. PMC: 554401. DOI: 10.1002/j.1460-2075.1985.tb03834.x. View

15.

Hudson G, Holton T, Whitfield P, Bottomley W . Spinach chloroplast rpoBC genes encode three subunits of the chloroplast RNA polymerase. J Mol Biol. 1988; 200(4):639-54. DOI: 10.1016/0022-2836(88)90477-9. View

16.

Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T . The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J. 1986; 5(9):2043-2049. PMC: 1167080. DOI: 10.1002/j.1460-2075.1986.tb04464.x. View

17.

Stirdivant S, Crossland L, Bogorad L . DNA supercoiling affects in vitro transcription of two maize chloroplast genes differently. Proc Natl Acad Sci U S A. 1985; 82(15):4886-90. PMC: 390462. DOI: 10.1073/pnas.82.15.4886. View

18.

Matsudaira P . Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987; 262(21):10035-8. View

19.

Bradley D, Gatenby A . Mutational analysis of the maize chloroplast ATPase-beta subunit gene promoter: the isolation of promoter mutants in E. coli and their characterization in a chloroplast in vitro transcription system. EMBO J. 1985; 4(13B):3641-8. PMC: 554712. DOI: 10.1002/j.1460-2075.1985.tb04129.x. View

20.

Bottomley W, Smith H, Bogorad L . RNA polymerases of maize: partial purification and properties of the chloroplast enzyme. Proc Natl Acad Sci U S A. 1971; 68(10):2412-6. PMC: 389433. DOI: 10.1073/pnas.68.10.2412. View