HPLC-based Assays for Enzymes of Glutathione Biosynthesis

Overview

Journal Curr Protoc Toxicol

Specialty Toxicology

Date 2012 Oct 10

PMID 23045057

Citations 5

Authors

C C White

C J Krejsa

D L Eaton

T J Kavanagh

Affiliations

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Abstract

Glutamate cysteine ligase and glutathione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.

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