American Ginseng Inhibits Vascular Smooth Muscle Cell Proliferation Via Suppressing Jak/Stat Pathway

Overview

Journal J Ethnopharmacol

Date 2012 Oct 9

PMID 23041701

Citations 7

Authors

Qi Wu

Wenjuan Wang

Siying Li

Prakash Nagarkatti

Mitzi Nagarkatti

Anthony Windust

Xing Li Wang

Dongqi Tang

Taixing Cui

Affiliations

Soon will be listed here.

Abstract

Unlabelled: ETHNOPHARMCOLOGICAL RELEVANCE: Ginseng, a folk medicine which has been used for thousands of years in Asia, has been promoted for the treatment or prevention of health problems including cardiovascular disease. However, the molecular mechanism of ginseng-induced cardiovascular protection is unclear. Thus, we investigated signaling mechanism by which American ginseng inhibits vascular smooth muscle cell (VSMC) proliferation, a key feature of diverse vascular disease.

Materials And Methods: A standardized crude extract of American ginseng was supplied by the National Research Council of Canada, Institute for National Measurement Standards. Rat aortic smooth muscle cells (RASMCs) were exposed to fetal bovine serum (FBS), platelet derived growth factor (PDGF), insulin, or angiotensin II (Ang II) to induce proliferation that was examined by measuring DNA synthesis and cell numbers. Western blot was applied to determine the activations of Jak, Stat, Akt, and ERK.

Results: American ginseng inhibited RASMC proliferation induced by FBS, PDGF, insulin or Ang II. American ginseng slightly increased both basal and FBS-, PDGF- or Ang II-induced activities of Akt and ERK in RASMCs; however, it dramatically inhibited the activation of Jak2 and Stat3.

Conclusion: These results demonstrate that American ginseng inhibits VSMC proliferation through suppressing the Jak/Stat pathway.

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References

Ichikawa T, Li J, Nagarkatti P, Nagarkatti M, Hofseth L, Windust A . American ginseng preferentially suppresses STAT/iNOS signaling in activated macrophages. J Ethnopharmacol. 2009; 125(1):145-50. PMC: 2790430. DOI: 10.1016/j.jep.2009.05.032. View

Rosamond W, Flegal K, Furie K, Go A, Greenlund K, Haase N . Heart disease and stroke statistics--2008 update: a report from the American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Circulation. 2007; 117(4):e25-146. DOI: 10.1161/CIRCULATIONAHA.107.187998. View

Zhang W, Chen G, Deng C . Effects and mechanisms of total Panax notoginseng saponins on proliferation of vascular smooth muscle cells with plasma pharmacology method. J Pharm Pharmacol. 2011; 64(1):139-45. DOI: 10.1111/j.2042-7158.2011.01379.x. View

Kim N, Kim E, Kang K, Cho M, Choi S, Kim S . Ginsenoside Rg3 inhibits phenylephrine-induced vascular contraction through induction of nitric oxide synthase. Br J Pharmacol. 2003; 140(4):661-70. PMC: 1574077. DOI: 10.1038/sj.bjp.0705490. View

Muto A, Fitzgerald T, Pimiento J, Maloney S, Teso D, Paszkowiak J . Smooth muscle cell signal transduction: implications of vascular biology for vascular surgeons. J Vasc Surg. 2007; 45 Suppl A:A15-24. PMC: 1939976. DOI: 10.1016/j.jvs.2007.02.061. View

GILLIS C . Panax ginseng pharmacology: a nitric oxide link?. Biochem Pharmacol. 1997; 54(1):1-8. DOI: 10.1016/s0006-2952(97)00193-7. View

Cui T, Nakagami H, Iwai M, Takeda Y, Shiuchi T, Tamura K . ATRAP, novel AT1 receptor associated protein, enhances internalization of AT1 receptor and inhibits vascular smooth muscle cell growth. Biochem Biophys Res Commun. 2001; 279(3):938-41. DOI: 10.1006/bbrc.2000.4055. View

Lee T, Johnke R, Allison R, OBrien K, Dobbs Jr L . Radioprotective potential of ginseng. Mutagenesis. 2005; 20(4):237-43. DOI: 10.1093/mutage/gei041. View

Lu J, Yao Q, Chen C . Ginseng compounds: an update on their molecular mechanisms and medical applications. Curr Vasc Pharmacol. 2009; 7(3):293-302. PMC: 2928028. DOI: 10.2174/157016109788340767. View

10.

Izzo A, Di Carlo G, Borrelli F, Ernst E . Cardiovascular pharmacotherapy and herbal medicines: the risk of drug interaction. Int J Cardiol. 2005; 98(1):1-14. DOI: 10.1016/j.ijcard.2003.06.039. View

11.

Kirabo A, Oh S, Kasahara H, Wagner K, Sayeski P . Vascular smooth muscle Jak2 deletion prevents angiotensin II-mediated neointima formation following injury in mice. J Mol Cell Cardiol. 2011; 50(6):1026-34. PMC: 3092026. DOI: 10.1016/j.yjmcc.2011.03.005. View

12.

Ammarguellat F, Llovera M, Kelly P, Goffin V . Low doses of EPO activate MAP kinases but not JAK2-STAT5 in rat vascular smooth muscle cells. Biochem Biophys Res Commun. 2001; 284(4):1031-8. DOI: 10.1006/bbrc.2001.5085. View

13.

Lee J, Lim K, Kim S, Bae O, Noh J, Chung S . Vascular smooth muscle dysfunction and remodeling induced by ginsenoside Rg3, a bioactive component of ginseng. Toxicol Sci. 2010; 117(2):505-14. DOI: 10.1093/toxsci/kfq201. View

14.

Chen D, Zuo G, Li C, Hu X, Guan T, Jiang R . Total saponins of Panax ginseng (TSPG) promote erythroid differentiation of human CD34+ cells via EpoR-mediated JAK2/STAT5 signaling pathway. J Ethnopharmacol. 2009; 126(2):215-20. DOI: 10.1016/j.jep.2009.08.043. View

15.

Li J, Ichikawa T, Jin Y, Hofseth L, Nagarkatti P, Nagarkatti M . An essential role of Nrf2 in American ginseng-mediated anti-oxidative actions in cardiomyocytes. J Ethnopharmacol. 2010; 130(2):222-30. PMC: 3920583. DOI: 10.1016/j.jep.2010.03.040. View

16.

Yu X, Deng J, Yang D, Gao Y, Gong Q, Huang X . Total Ginsenosides suppress the neointimal hyperplasia of rat carotid artery induced by balloon injury. Vascul Pharmacol. 2010; 54(1-2):52-7. DOI: 10.1016/j.vph.2010.12.003. View

17.

Wang T, Yu X, Qu S, Xu H, Sui D . Ginsenoside Rb3 inhibits angiotensin II-induced vascular smooth muscle cells proliferation. Basic Clin Pharmacol Toxicol. 2010; 107(2):685-9. DOI: 10.1111/j.1742-7843.2010.00560.x. View

18.

Xu L, Liu J, Liu N, Lu P, Pang X . Effects of Panax notoginseng saponins on proliferation and apoptosis of vascular smooth muscle cells. J Ethnopharmacol. 2011; 137(1):226-30. DOI: 10.1016/j.jep.2011.05.020. View

19.

Wu C, Tsai B, Hsieh W, Chang G, Mao S, Chang W . The preventive effects of G115 on balloon injury-induced neointima formation in rats. Life Sci. 2002; 70(6):669-79. DOI: 10.1016/s0024-3205(01)01442-4. View

20.

Zhang H, Wang S . Ginsenoside Rg1 inhibits tumor necrosis factor-alpha (TNF-alpha)-induced human arterial smooth muscle cells (HASMCs) proliferation. J Cell Biochem. 2006; 98(6):1471-81. DOI: 10.1002/jcb.20799. View