Heterologous Production of the Lantibiotic Ala(0)actagardine in Escherichia Coli

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2012 Oct 5

PMID 23034674

Citations 21

Authors

Yanxiang Shi

Alejandro Bueno

Wilfred A van der Donk

Affiliations

Soon will be listed here.

Abstract

We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.

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