Amphiphilic Graft Copolymer Based on Poly(styrene-co-maleic Anhydride) with Low Molecular Weight Polyethylenimine for Efficient Gene Delivery

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2012 Oct 3

PMID 23028224

Citations 9

Authors

Xiaopin Duan

Jisheng Xiao

Qi Yin

Zhiwen Zhang

Shirui Mao

Yaping Li

Affiliations

Soon will be listed here.

Abstract

Background And Methods: A new amphiphilic comb-shaped copolymer (SP) was synthesized by conjugating poly(styrene-co-maleic anhydride) with low molecular weight polyethyleneimine for gene delivery. Fourier transform infrared spectrum, (1)H nuclear magnetic resonance, and gel permeation chromatography were used to characterize the graft copolymer.

Results: The buffering capability of SP was similar to that of polyethyleneimine within the endosomal pH range. The copolymer could condense DNA effectively to form complexes with a positive charge (13-30 mV) and a small particle size (130-200 nm) at N/P ratios between 5 and 20, and protect DNA from degradation by DNase I. In addition, SP showed much lower cytotoxicity than polyethyleneimine 25,000. Importantly, the gene transfection activity and cellular uptake of SP-DNA complexes were all markedly higher than that of complexes of polyethyleneimine 25,000 and DNA in MCF-7 and MCF-7/ADR cell lines.

Conclusion: This work highlights the promise of SP as a safe and efficient synthetic vector for DNA delivery.

Citing Articles

Preparation and characterization of calcium-doped graphene oxide-chitosan Nanocarrier to enhance the gene delivery in MCF-7 cell line.

Bidgoli A, Farmany A, Taheri M, Soleimani M, Nouri F Sci Rep. 2024; 14(1):27434.

PMID: 39521829 PMC: 11550409. DOI: 10.1038/s41598-024-78958-3.

One-Step Synthesis of Polyethyleneimine-Grafted Styrene-Maleic Anhydride Copolymer Adsorbents for Effective Adsorption of Anionic Dyes.

Xu Y, Wang Q, Wang Y, Hu F, Sun B, Gao T Molecules. 2024; 29(8).

PMID: 38675707 PMC: 11054579. DOI: 10.3390/molecules29081887.

Fabrication of Eco-Friendly Hydrolyzed Ethylene-Maleic Anhydride Copolymer-Avermectin Nanoemulsion with High Stability, Adhesion Property, pH, and Temperature-Responsive Releasing Behaviors.

Cheng Y, Pan Z, Tang L, Huang Y, Yang W Molecules. 2024; 29(5).

PMID: 38474660 PMC: 10934311. DOI: 10.3390/molecules29051148.

Polymer encapsulated clinical ICG nanoparticles for enhanced photothermal therapy and NIR fluorescence imaging in cervical cancer.

Chen S, Zhu L, Du Z, Ma R, Yan T, Alimu G RSC Adv. 2022; 11(34):20850-20858.

PMID: 35479387 PMC: 9034030. DOI: 10.1039/d1ra02875h.

Colorimetric Visualization Using Polymeric Core-Shell Nanoparticles: Enhanced Sensitivity for Formaldehyde Gas Sensors.

Park J, Kim Y, Lee C, Kook J, Kim D, Kim J Polymers (Basel). 2020; 12(5).

PMID: 32344883 PMC: 7285312. DOI: 10.3390/polym12050998.

References

Wen Y, Pan S, Luo X, Zhang X, Zhang W, Feng M . A biodegradable low molecular weight polyethylenimine derivative as low toxicity and efficient gene vector. Bioconjug Chem. 2009; 20(2):322-32. DOI: 10.1021/bc800428y. View

Liu K, Wang X, Fan W, Zhu Q, Yang J, Gao J . Degradable polyethylenimine derivate coupled to a bifunctional peptide R13 as a new gene-delivery vector. Int J Nanomedicine. 2012; 7:1149-62. PMC: 3299202. DOI: 10.2147/IJN.S28819. View

Chen X, Zhang L, He Z, Wang W, Xu B, Zhong Q . Plasmid-encapsulated polyethylene glycol-grafted polyethylenimine nanoparticles for gene delivery into rat mesenchymal stem cells. Int J Nanomedicine. 2011; 6:843-53. PMC: 3090281. DOI: 10.2147/IJN.S17155. View

Pack D, Hoffman A, Pun S, Stayton P . Design and development of polymers for gene delivery. Nat Rev Drug Discov. 2005; 4(7):581-93. DOI: 10.1038/nrd1775. View

Lin J, Hsu Y . Temperature and pH-responsive properties of poly(styrene-co-maleic anhydride)-grafting poly(oxypropylene)-amines. J Colloid Interface Sci. 2009; 336(1):82-9. DOI: 10.1016/j.jcis.2009.03.064. View

Neu M, Fischer D, Kissel T . Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives. J Gene Med. 2005; 7(8):992-1009. DOI: 10.1002/jgm.773. View

Iwai K, Maeda H, Konno T . Use of oily contrast medium for selective drug targeting to tumor: enhanced therapeutic effect and X-ray image. Cancer Res. 1984; 44(5):2115-21. View

Boussif O, Lezoualch F, Zanta M, Mergny M, Scherman D, Demeneix B . A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A. 1995; 92(16):7297-301. PMC: 41326. DOI: 10.1073/pnas.92.16.7297. View

Li D, Ping Y, Xu F, Yu H, Pan H, Huang H . Construction of a star-shaped copolymer as a vector for FGF receptor-mediated gene delivery in vitro and in vivo. Biomacromolecules. 2010; 11(9):2221-9. DOI: 10.1021/bm100141y. View

10.

Chabaud P, Camplo M, Payet D, Serin G, Moreau L, Barthelemy P . Cationic nucleoside lipids for gene delivery. Bioconjug Chem. 2006; 17(2):466-72. DOI: 10.1021/bc050162q. View

11.

Zovko M, Barbaric M, Zorc B, Hafner A, Filipovic-Grcic J . Synthesis of fenoprofen and gemfibrozil styrene-maleic acid copolymer conjugates. Acta Pharm. 2005; 55(2):169-76. View

12.

WINEK C, Burgun J . Acute and subacute toxicology and safety evaluation of SMA 1440-H resin. Clin Toxicol. 1977; 10(2):255-60. DOI: 10.3109/15563657708987970. View

13.

Mu Y, Kamada H, Kaneda Y, Yamamoto Y, Kodaira H, Tsunoda S . Bioconjugation of laminin peptide YIGSR with poly(styrene co-maleic acid) increases its antimetastatic effect on lung metastasis of B16-BL6 melanoma cells. Biochem Biophys Res Commun. 1999; 255(1):75-9. DOI: 10.1006/bbrc.1999.9930. View

14.

Clamme J, VUILLEUMIER C, Kuhry J, Mely Y . Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes. Biochim Biophys Acta. 2001; 1514(1):21-32. DOI: 10.1016/s0005-2736(01)00359-5. View

15.

Maeda H . SMANCS and polymer-conjugated macromolecular drugs: advantages in cancer chemotherapy. Adv Drug Deliv Rev. 2001; 46(1-3):169-85. DOI: 10.1016/s0169-409x(00)00134-4. View

16.

Wang Y, Su J, Wu F, Lu P, Yuan L, Yuan W . Biscarbamate cross-linked polyethylenimine derivative with low molecular weight, low cytotoxicity, and high efficiency for gene delivery. Int J Nanomedicine. 2012; 7:693-704. PMC: 3282609. DOI: 10.2147/IJN.S27849. View

17.

Zhu J, Tang A, Law L, Feng M, Ho K, Lee D . Amphiphilic core-shell nanoparticles with poly(ethylenimine) shells as potential gene delivery carriers. Bioconjug Chem. 2005; 16(1):139-46. DOI: 10.1021/bc049895l. View

18.

Kim Y, Park J, Lee M, Kim Y, Park T, Kim S . Polyethylenimine with acid-labile linkages as a biodegradable gene carrier. J Control Release. 2005; 103(1):209-19. DOI: 10.1016/j.jconrel.2004.11.008. View

19.

Liu J, Jiang X, Xu L, Wang X, Hennink W, Zhuo R . Novel reduction-responsive cross-linked polyethylenimine derivatives by click chemistry for nonviral gene delivery. Bioconjug Chem. 2010; 21(10):1827-35. DOI: 10.1021/bc100191r. View

20.

Park T, Jeong J, Kim S . Current status of polymeric gene delivery systems. Adv Drug Deliv Rev. 2006; 58(4):467-86. DOI: 10.1016/j.addr.2006.03.007. View