A Sandwich-type DNA Electrochemical Biosensor for Hairpin-stem-loop Structure Based on Multistep Temperature-controlling Method

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2012 Oct 3

PMID 23028223

Citations 1

Authors

Guolin Hong

Yinhuan Liu

Wei Chen

Shaohuang Weng

Qicai Liu

Ailin Liu

Daoxin Zheng

Xinhua Lin

Affiliations

Soon will be listed here.

Abstract

A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection.

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