Use of an Improved Quantitative Polymerase Chain Reaction Assay to Determine Differences in Human Rhinovirus Viral Loads in Different Populations

Overview

Journal Diagn Microbiol Infect Dis

Specialties Infectious Diseases
Microbiology
Pathology

Date 2012 Sep 29

PMID 23017257

Citations 12

Authors

Andrea Granados

Kathy Luinstra

Sylvia Chong

Emma Goodall

Lisa Banh

Samira Mubareka

Marek Smieja

James Mahony

Affiliations

Soon will be listed here.

Abstract

Human rhinoviruses (HRV) frequently cause acute respiratory infections and chronic respiratory disease exacerbations. However, testing is not generally offered. We developed a modified HRV quantitative polymerase chain reaction (qPCR) assay to assess viral loads in the community and hospital patients. The assay had a lower limit of detection of 2 log(10) viral copies/mL and displayed linearity over 5 log(10) viral copies, with a lower limit of quantitation of 4 log(10) viral copies/mL. Mean viral loads (95% confidence interval) for hospitalized children, university students, and institutionalized elderly, were 7.08 log(10) viral copies/mL (6.7-7.5), 6.87 log(10) viral copies/mL (6.5-7.2), and 7.09 log(10) viral copies/mL (6.9-7.3), respectively (P = 0.67). Serial specimens of 14 university students showed a decrease of mean viral loads from 6.36 log(10) viral copies/mL on day 1 to 2.32 log(10) viral copies/mL 7 days past symptom onset (P < 0.001). Using an HRV qPCR, we showed that viral loads did not differ between the community and hospitalized populations and significantly decreased following symptoms onset in healthy individuals.

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