Expression of TRAF6 and Ubiquitin MRNA in Skeletal Muscle of Gastric Cancer Patients

Overview

Journal J Exp Clin Cancer Res

Publisher Biomed Central

Specialty Oncology

Date 2012 Sep 28

PMID 23013936

Citations 22

Authors

Yuan-shui Sun

Zai-Yuan Ye

Zhen-Yuan Qian

Xiao-Dong Xu

Jun-Feng Hu

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the prognostic significance of tumor necrosis factor receptor (TNFR),-associated factor 6 (TRAF6),-and ubiquitin in gastric cancer patients.

Methods: Biopsies of the rectus abdominis muscle were obtained intra operatively from 102 gastric cancer patients and 29 subjects undergoing surgery for benign abdominal diseases, and muscle TRAF6 and ubiquitin mRNA expression and proteasome proteolytic activities were assessed.

Results: TRAF6 was significantly upregulated in muscle of gastric cancer compared with the control muscles. TRAF6 was upregulated in 67.65% (69/102) muscle of gastric cancer. Over expression of TRAF6 in muscles of gastric cancer were associated with TNM stage, level of serum albumin and percent of weight loss. Ubiquitin was significantly upregulated in muscle of gastric cancer compared with the control muscles. Ubiquitin was upregulated in 58.82% (60/102) muscles of gastric cancer. Over expression of ubiquitin in muscles of gastric cancer were associated with TNM (Tumor-Node-Metastasis) stage and weight loss. There was significant relation between TRAF6 and ubiquitin expression.

Conclusions: We found a positive correlation between TRAF6 and ubiquitin expression, suggesting that TRAF6 may up regulates ubiquitin activity in cancer cachexia. While more investigations are required to understand its mechanisms of TRAF6 and ubiquitin in skeletal muscle. Correct the catabolic-anabolic imbalance is essential for the effective treatment of cancer cachexia.

Citing Articles

Influence of Amino Acids and Exercise on Muscle Protein Turnover, Particularly in Cancer Cachexia.

Pradhan R, Dieterich W, Natarajan A, Schwappacher R, Reljic D, Herrmann H Cancers (Basel). 2024; 16(10).

PMID: 38791998 PMC: 11119313. DOI: 10.3390/cancers16101921.

Molecular mechanisms of cancer cachexia-related loss of skeletal muscle mass: data analysis from preclinical and clinical studies.

Martin A, Gallot Y, Freyssenet D J Cachexia Sarcopenia Muscle. 2023; 14(3):1150-1167.

PMID: 36864755 PMC: 10235899. DOI: 10.1002/jcsm.13073.

The role of long non-coding RNA HCG18 in cancer.

Wang Z, Ran R, Zhang S, Zhou W, Lv J, Ma C Clin Transl Oncol. 2022; 25(3):611-619.

PMID: 36346572 DOI: 10.1007/s12094-022-02992-8.

Long non-coding RNA HCG18 promotes gastric cancer progression by regulating miRNA-146a-5p/tumor necrosis factor receptor-associated factor 6 axis.

Yang X, Liu R Bioengineered. 2022; 13(3):6781-6793.

PMID: 35240920 PMC: 8973972. DOI: 10.1080/21655979.2022.2034565.

Revisiting Cancer Cachexia: Pathogenesis, Diagnosis, and Current Treatment Approaches.

Baba M, Buch S Asia Pac J Oncol Nurs. 2021; 8(5):508-518.

PMID: 34527780 PMC: 8420916. DOI: 10.4103/apjon.apjon-2126.

References

Kubrak C, Olson K, Jha N, Jensen L, McCargar L, Seikaly H . Nutrition impact symptoms: key determinants of reduced dietary intake, weight loss, and reduced functional capacity of patients with head and neck cancer before treatment. Head Neck. 2009; 32(3):290-300. DOI: 10.1002/hed.21174. View

Baracos V . Cancer-associated cachexia and underlying biological mechanisms. Annu Rev Nutr. 2006; 26:435-61. DOI: 10.1146/annurev.nutr.26.061505.111151. View

Bossola M, Muscaritoli M, Costelli P, Grieco G, Bonelli G, Pacelli F . Increased muscle proteasome activity correlates with disease severity in gastric cancer patients. Ann Surg. 2003; 237(3):384-9. PMC: 1514311. DOI: 10.1097/01.SLA.0000055225.96357.71. View

Chopard A, Hillock S, Jasmin B . Molecular events and signalling pathways involved in skeletal muscle disuse-induced atrophy and the impact of countermeasures. J Cell Mol Med. 2009; 13(9B):3032-50. PMC: 4516463. DOI: 10.1111/j.1582-4934.2009.00864.x. View

Gullett N, Rossi P, Kucuk O, Johnstone P . Cancer-induced cachexia: a guide for the oncologist. J Soc Integr Oncol. 2009; 7(4):155-69. View

Paul P, Gupta S, Bhatnagar S, Panguluri S, Darnay B, Choi Y . Targeted ablation of TRAF6 inhibits skeletal muscle wasting in mice. J Cell Biol. 2010; 191(7):1395-411. PMC: 3010064. DOI: 10.1083/jcb.201006098. View

Kumar A, Bhatnagar S, Paul P . TWEAK and TRAF6 regulate skeletal muscle atrophy. Curr Opin Clin Nutr Metab Care. 2012; 15(3):233-9. PMC: 3397822. DOI: 10.1097/MCO.0b013e328351c3fc. View

Dodson S, Baracos V, Jatoi A, Evans W, Cella D, Dalton J . Muscle wasting in cancer cachexia: clinical implications, diagnosis, and emerging treatment strategies. Annu Rev Med. 2010; 62:265-79. DOI: 10.1146/annurev-med-061509-131248. View

Evans W, Morley J, Argiles J, Bales C, Baracos V, Guttridge D . Cachexia: a new definition. Clin Nutr. 2008; 27(6):793-9. DOI: 10.1016/j.clnu.2008.06.013. View

10.

Tisdale M . Mechanisms of cancer cachexia. Physiol Rev. 2009; 89(2):381-410. DOI: 10.1152/physrev.00016.2008. View

11.

Baracos V . Management of muscle wasting in cancer-associated cachexia: understanding gained from experimental studies. Cancer. 2001; 92(6 Suppl):1669-77. DOI: 10.1002/1097-0142(20010915)92:6+<1669::aid-cncr1495>3.0.co;2-s. View

12.

Evans W . Skeletal muscle loss: cachexia, sarcopenia, and inactivity. Am J Clin Nutr. 2010; 91(4):1123S-1127S. DOI: 10.3945/ajcn.2010.28608A. View

13.

Onodera T, Goseki N, KOSAKI G . [Prognostic nutritional index in gastrointestinal surgery of malnourished cancer patients]. Nihon Geka Gakkai Zasshi. 1984; 85(9):1001-5. View

14.

Paul P, Kumar A . TRAF6 coordinates the activation of autophagy and ubiquitin-proteasome systems in atrophying skeletal muscle. Autophagy. 2011; 7(5):555-6. PMC: 3127217. DOI: 10.4161/auto.7.5.15102. View