Erythropoietin Ameliorates Oxidative Stress and Tissue Injury Following Renal Ischemia/reperfusion in Rat Kidney and Lung

Overview

Journal Med Princ Pract

Publisher Karger

Specialties General Medicine
Medical Education

Date 2012 Sep 26

PMID 23006583

Citations 14

Authors

Mohammad Reza Ardalan

Rasoul Estakhri

Babak Hajipour

Khalil Ansarin

Naser Ahmadi Asl

Mohammad Reza Nasirizade

Alireza Nour Azar

Amir Ghorbanihaghjou

Amir Mansour Vatankhah

Heydar Ali Esmaili

Affiliations

Soon will be listed here.

Abstract

Objective: To study the effect of erythropoietin (EPO) treatment on renal and lung injury following renal ischemia/reperfusion (I/R).

Materials And Methods: Thirty male Wistar rats were assigned to three groups of 10 rats each. The first group was sham-operated, the second was subjected to renal I/R (30 min of ischemia followed by 24 h of reperfusion). The third group was subjected to renal I/R and treated with EPO in two doses: the first dose 1 h prior to ischemia (1,000 U/kg) and the second dose 6 h after ischemia (1,000 U/kg).

Results: The renal and lung tissue injury index, tissue serum blood urea nitrogen and creatinine (Cr) were higher in the renal I/R group compared to the renal I/R + EPO group; the difference was statistically significant (p < 0.05). Kidney and lung tissue glutathione peroxidase and superoxide dismutase levels were higher in the renal I/R + EPO group than the renal I/R group; the difference was also statistically significant (p < 0.05).

Conclusion: The data showed that EPO pretreatment could be effective in reducing renal and lung injury following renal I/R and could improve the cellular antioxidant defense system. Hence EPO pretreatment may be effective for attenuating renal and lung injury after renal I/R-induced injury during surgical procedures, hypotension, renal transplantation and other conditions inducing renal I/R.

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