Escherichia Coli-mediated Impairment of Ureteric Contractility is Uropathogenic E. Coli Specific

Overview

Journal J Infect Dis

Publisher Oxford University Press

Specialty Infectious Diseases

Date 2012 Sep 25

PMID 23002447

Citations 11

Authors

Rachel V Floyd

Mathew Upton

Scott J Hultgren

Susan Wray

Theodor V Burdyga

Craig Winstanley

Affiliations

Soon will be listed here.

Abstract

Background: Ureters are fundamental for keeping kidneys free from uropathogenic Escherichia coli (UPEC), but we have shown that 2 strains (J96 and 536) can subvert this role and reduce ureteric contractility. To determine whether this is (1) a widespread feature of UPEC, (2) exhibited only by UPEC, and (3) dependent upon type 1 fimbriae, we analyzed strains representing epidemiologically important multilocus sequence types ST131, ST73, and ST95 and non-UPEC E. coli.

Methods: Contractility and calcium transients in intact rat ureters were compared between strains. Mannose and fim mutants were used to investigate the role of type 1 fimbriae.

Results: Non-UPEC had no significant effect on contractility, with a mean decrease after 8 hours of 8.8%, compared with 8.8% in controls. UPEC effects on contractility were strain specific, with decreases from 9.47% to 96.7%. Mannose inhibited the effects of the most potent strains (CFT073 and UTI89) but had variable effects among other UPEC strains. Mutation and complementation studies showed that the effects of the UTI89 cystitis isolate were fimH dependent.

Conclusions: We find that (1) non-UPEC do not affect ureteric contractility, (2) impairment of contractility is a common feature of UPEC, and (3) the mechanism varies between strains, but for the most potent UPEC type 1 fimbriae are involved.

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Invasion and diversity in urinary tract infections.

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Innate Bacteriostatic Mechanisms Defend the Urinary Tract.

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Plasmids shape the diverse accessory resistomes of ST131.

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Forde B, Phan M, Gawthorne J, Ashcroft M, Stanton-Cook M, Sarkar S mBio. 2015; 6(6):e01602-15.

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