Endothelial Differentiation in Multipotent Cells Derived from Mouse and Human White Mature Adipocytes

Overview

Journal J Mol Cell Cardiol

Specialty Cardiology & Vascular Diseases

Date 2012 Sep 25

PMID 22999861

Citations 29

Authors

Medet Jumabay

Raushan Abdmaulen

Sumithra Urs

Sepideh Heydarkhan-Hagvall

Gregorio D Chazenbalk

Maria C Jordan

Kenneth P Roos

Yucheng Yao

Kristina I Bostrom

Affiliations

Soon will be listed here.

Abstract

White mature adipocytes give rise to multipotent cells, so-called de-differentiated fat (DFAT) cells, when losing their fat in culture. The objective of this study was to examine the ability of DFAT cells to give rise to endothelial cells (ECs) in vitro and vivo. We demonstrate that mouse and human DFAT cells, derived from adipose tissue and lipospirate, respectively, initially lack expression of CD34, CD31, CD146, CD45 and pericyte markers, distinguishing them from progenitor cells previously identified in adipose stroma. The DFAT cells spontaneously differentiate into vascular ECs in vitro, as determined by real-time PCR, fluorescence activated cell sorting, immunostaining, and formation of tube structures. Treatment with bone morphogenetic protein (BMP)4 and BMP9, important in regulating angiogenesis, significantly enhances the EC differentiation. Furthermore, adipocyte-derived cells from Green Fluorescent Protein-transgenic mice were detected in the vasculature of infarcted myocardium up to 6 weeks after ligation of the left anterior descending artery in mice. We conclude that adipocyte-derived multipotent cells are able to spontaneously give rise to ECs, a process that is promoted by BMPs and may be important in cardiovascular regeneration and in physiological and pathological changes in fat and other tissues.

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