CHOP and Caspase 3 Induction Underlie Glioblastoma Cell Death in Response to Endoplasmic Reticulum Stress

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2012 Sep 13

PMID 22969916

Citations 14

Authors

Quincy A Quick

Milton O Faison

Affiliations

Soon will be listed here.

Abstract

The unfolded protein endoplasmic reticulum stress response has emerged as a cellular physiological target to invoke tumor cell killing due to its homeostatic and cytoprotective functions. In this study, thapsigargin and tunicamycin, two endoplasmic reticulum stress inducers, were investigated for their efficacy on glioblastomas. We demonstrate that clinically relevant concentrations of thapsigargin and tunicamycin eliminate the glioblastoma cell reproductive capacity as a consequence of cell death. The mode of glioblastoma-induced cell death was determined to be via apoptosis as supported by increased C/EBP homologous protein (CHOP) levels and caspase 3 activity, two proteins with established roles in endoplasmic reticulum stress-induced cell death. In conclusion, this study provides evidence that glioblastomas are responsive to endoplasmic reticulum stress induction as a cellular program to eradicate this tumor via programmed cell death.

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