A Novel Reproductive Peptide, Phoenixin

Overview

Journal J Neuroendocrinol

Specialty Endocrinology

Date 2012 Sep 12

PMID 22963497

Citations 66

Authors

G L C Yosten

R-M Lyu

A J W Hsueh

O Avsian-Kretchmer

J-K Chang

C W Tullock

S L Dun

N Dun

W K Samson

Affiliations

Soon will be listed here.

Abstract

Normal anterior pituitary function is essential for fertility. Release from the gland of the reproductive hormones luteinising hormone and follicle-stimulating hormone is regulated primarily by hypothalamically-derived gonadotrophin-releasing hormone (GnRH), although other releasing factors (RF) have been postulated to exist. Using a bioinformatic approach, we have identified a novel peptide, phoenixin, that regulates pituitary gonadotrophin secretion by modulating the expression of the GnRH receptor, an action with physiologically relevant consequences. Compromise of phoenixin in vivo using small interfering RNA resulted in the delayed appearance of oestrus and a reduction in GnRH receptor expression in the pituitary. Phoenixin may represent a new class of hypothalamically-derived pituitary priming factors that sensitise the pituitary to the action of other RFs, rather than directly stimulating the fusion of secretary vesicles to pituitary membranes.

Citing Articles

Protective Effects of Phoenixin-14 Administration Against Renal Ischemia/Reperfusion Injury in Rats.

Oz S, Bahar M, Sekerci G, Taslidere A, Tekin S J Biochem Mol Toxicol. 2025; 39(3):e70200.

PMID: 40025799 PMC: 11873675. DOI: 10.1002/jbt.70200.

Immunolocalization and quantification of the phoenixin and GPR173 in the gastrointestinal tract of Holstein-Friesian bulls.

Kras K, Osiak-Wicha C, Arciszewski M BMC Vet Res. 2025; 21(1):76.

PMID: 39966825 PMC: 11834677. DOI: 10.1186/s12917-025-04545-x.

Potential Modulatory Role of Phoenixin-14 in Epithelial-Mesenchymal Transition of Endometriotic 12Z Cells.

Kulinska K, Wierzbicka M, Dera-Szymanowska A, Szymanowski K, Andrusiewicz M, Wolun-Cholewa M Biomedicines. 2025; 13(1).

PMID: 39857742 PMC: 11762346. DOI: 10.3390/biomedicines13010158.

Spatial and quantitative gene expression analysis of SREB receptors in the gonads of green-spotted pufferfish (Dichotomyctere nigroviridis).

Breton T, Oliveira M, Chillemi T, Harriman W, Korasadowicz J, Saverese E Gen Comp Endocrinol. 2024; 360:114641.

PMID: 39536984 PMC: 11646178. DOI: 10.1016/j.ygcen.2024.114641.

Nesfatin-1 expression and blood plasma concentration in female dogs suffering from cystic endometrial hyperplasia and pyometra and its possible interaction with phoenixin-14.

Rybska M, Skrzypski M, Billert M, Wojciechowicz T, Lukomska A, Pawlak P BMC Vet Res. 2024; 20(1):486.

PMID: 39455994 PMC: 11520108. DOI: 10.1186/s12917-024-04336-w.

References

Vasilyev V, Pernasetti F, Rosenberg S, Barsoum M, Austin D, Webster N . Transcriptional activation of the ovine follicle-stimulating hormone-beta gene by gonadotropin-releasing hormone involves multiple signal transduction pathways. Endocrinology. 2002; 143(5):1651-9. PMC: 2930615. DOI: 10.1210/endo.143.5.8771. View

McCANN S, Lumpkin M, Mizunuma H, Khorram O, Samson W . Recent studies on the role of brain peptides in control of anterior pituitary hormone secretion. Peptides. 1984; 5 Suppl 1:3-7. DOI: 10.1016/0196-9781(84)90259-6. View

Levine J, Ramirez V . Luteinizing hormone-releasing hormone release during the rat estrous cycle and after ovariectomy, as estimated with push-pull cannulae. Endocrinology. 1982; 111(5):1439-48. DOI: 10.1210/endo-111-5-1439. View

Brann D, OConner J, Wade M, Zamorano P, Mahesh V . Regulation of anterior pituitary gonadotropin subunit mRNA levels during the preovulatory gonadotropin surge: a physiological role of progesterone in regulating LH-beta and FSH-beta mRNA levels. J Steroid Biochem Mol Biol. 1993; 46(4):427-37. DOI: 10.1016/0960-0760(93)90097-g. View

Clarke I, Cummins J . The temporal relationship between gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in ovariectomized ewes. Endocrinology. 1982; 111(5):1737-9. DOI: 10.1210/endo-111-5-1737. View

Schmittgen T, Livak K . Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008; 3(6):1101-8. DOI: 10.1038/nprot.2008.73. View

Burger L, Haisenleder D, Dalkin A, Marshall J . Regulation of gonadotropin subunit gene transcription. J Mol Endocrinol. 2004; 33(3):559-84. DOI: 10.1677/jme.1.01600. View

Samson W, Martin L, Mogg R, Fulton R . A nonoxytocinergic prolactin releasing factor and a nondopaminergic prolactin inhibiting factor in bovine neurointermediate lobe extracts: in vitro and in vivo studies. Endocrinology. 1990; 126(3):1610-7. DOI: 10.1210/endo-126-3-1610. View

Koda A, Ukena K, Teranishi H, Ohta S, Yamamoto K, Kikuyama S . A novel amphibian hypothalamic neuropeptide: isolation, localization, and biological activity. Endocrinology. 2002; 143(2):411-9. DOI: 10.1210/endo.143.2.8630. View

10.

Brothers K, Wu S, DiVall S, Messmer M, Kahn C, Miller R . Rescue of obesity-induced infertility in female mice due to a pituitary-specific knockout of the insulin receptor. Cell Metab. 2010; 12(3):295-305. PMC: 2935812. DOI: 10.1016/j.cmet.2010.06.010. View

11.

Dun S, Brailoiu G, Tica A, Yang J, Chang J, Brailoiu E . Neuronostatin is co-expressed with somatostatin and mobilizes calcium in cultured rat hypothalamic neurons. Neuroscience. 2010; 166(2):455-63. PMC: 2824007. DOI: 10.1016/j.neuroscience.2009.12.059. View

12.

Kaiser U, Jakubowiak A, Steinberger A, Chin W . Regulation of rat pituitary gonadotropin-releasing hormone receptor mRNA levels in vivo and in vitro. Endocrinology. 1993; 133(2):931-4. DOI: 10.1210/endo.133.2.8393779. View

13.

Windle J, Weiner R, Mellon P . Cell lines of the pituitary gonadotrope lineage derived by targeted oncogenesis in transgenic mice. Mol Endocrinol. 1990; 4(4):597-603. DOI: 10.1210/mend-4-4-597. View

14.

Vasilyev V, Lawson M, Dipaolo D, Webster N, Mellon P . Different signaling pathways control acute induction versus long-term repression of LHbeta transcription by GnRH. Endocrinology. 2002; 143(9):3414-26. PMC: 2932485. DOI: 10.1210/en.2001-211215. View

15.

Lerrant Y, Kottler M, Bergametti F, Moumni M, Counis R . Expression of gonadotropin-releasing hormone (GnRH) receptor gene is altered by GnRH agonist desensitization in a manner similar to that of gonadotropin beta-subunit genes in normal and castrated rat pituitary. Endocrinology. 1995; 136(7):2803-8. DOI: 10.1210/endo.136.7.7789305. View

16.

Samson W, McCANN S . Effects of lesions in the organum vasculosum lamina terminalis on the hypothalamic distribution of luteinizing hormone-releasing hormone and gonadotropin secretion in the ovariectomized rat. Endocrinology. 1979; 105(4):939-46. DOI: 10.1210/endo-105-4-939. View

17.

Samson W, Zhang J, Avsian-Kretchmer O, Cui K, Yosten G, Klein C . Neuronostatin encoded by the somatostatin gene regulates neuronal, cardiovascular, and metabolic functions. J Biol Chem. 2008; 283(46):31949-59. PMC: 2581552. DOI: 10.1074/jbc.M804784200. View

18.

Samson W, Said S, Snyder G, McCANN S . In vitro stimulation of prolactin release by vasoactive intestinal peptide. Peptides. 1980; 1(4):325-32. DOI: 10.1016/0196-9781(80)90010-8. View

19.

Daniel P . The blood supply of the hypothalamus and pituitary gland. Br Med Bull. 1966; 22(3):202-8. DOI: 10.1093/oxfordjournals.bmb.a070474. View

20.

Novaira H, Ng Y, Wolfe A, Radovick S . Kisspeptin increases GnRH mRNA expression and secretion in GnRH secreting neuronal cell lines. Mol Cell Endocrinol. 2009; 311(1-2):126-34. PMC: 3534746. DOI: 10.1016/j.mce.2009.06.011. View