Differential Gene Expression of Wnt Signaling Pathway in Benign, Premalignant, and Malignant Human Breast Epithelial Cells

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2012 Sep 4

PMID 22941468

Citations 4

Authors

Yu-Zhou Wang

Yu-Song Han

Yu-Shui Ma

Jun-Jian Jiang

Zi-Xian Chen

Yi-Chao Wang

Wu Che

Feng Zhang

Qing Xia

Xiao-Feng Wang

Affiliations

Soon will be listed here.

Abstract

To gain insight into the role of gene expression alterations in breast cancer progression, we conducted a comprehensive gene expression analysis of a series of cell lines derived from MCF10A, which include benign MCF10A cells, premalignant AT, and malignant CA1a tumor cells. We analyzed gene expression variation using the Agilent Human Genome Oligo Microarray with the goal of identifying gene-specific expression change events. In addition to a previously noted overexpression in oncogene MDM2, HRAS, and PCNA, our studies identified overexpression of Wnt signaling pathway in malignant breast cell lines. The Kaplan-Meier plot showed that high c-Myc expression in breast cancer was associated with tumor progression and the patient's poor survival. This study showed that the Wnt pathway has further provided a basis for the development of potential biomarker for breast cancer prognosis.

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References

Sadlonova A, Mukherjee S, Bowe D, Gault S, Dumas N, Van Tine B . Human breast fibroblasts inhibit growth of the MCF10AT xenograft model of proliferative breast disease. Am J Pathol. 2007; 170(3):1064-76. PMC: 1864888. DOI: 10.2353/ajpath.2007.060031. View

Strickland L, Dawson P, Santner S, Miller F . Progression of premalignant MCF10AT generates heterogeneous malignant variants with characteristic histologic types and immunohistochemical markers. Breast Cancer Res Treat. 2001; 64(3):235-40. DOI: 10.1023/a:1026562720218. View

Kadota M, Yang H, Gomez B, Sato M, Clifford R, Meerzaman D . Delineating genetic alterations for tumor progression in the MCF10A series of breast cancer cell lines. PLoS One. 2010; 5(2):e9201. PMC: 2821407. DOI: 10.1371/journal.pone.0009201. View

Al-Hajj M, Wicha M, Benito-Hernandez A, Morrison S, Clarke M . Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A. 2003; 100(7):3983-8. PMC: 153034. DOI: 10.1073/pnas.0530291100. View

Santner S, Dawson P, Tait L, SOULE H, Eliason J, Mohamed A . Malignant MCF10CA1 cell lines derived from premalignant human breast epithelial MCF10AT cells. Breast Cancer Res Treat. 2001; 65(2):101-10. DOI: 10.1023/a:1006461422273. View

Dawson P, WOLMAN S, Tait L, Heppner G, Miller F . MCF10AT: a model for the evolution of cancer from proliferative breast disease. Am J Pathol. 1996; 148(1):313-9. PMC: 1861604. View

Gyorffy B, Lanczky A, Eklund A, Denkert C, Budczies J, Li Q . An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Treat. 2009; 123(3):725-31. DOI: 10.1007/s10549-009-0674-9. View

Zhao H, Ho P, Lo Y, Espejo A, Bedford M, Hung M . Interaction of proliferation cell nuclear antigen (PCNA) with c-Abl in cell proliferation and response to DNA damages in breast cancer. PLoS One. 2012; 7(1):e29416. PMC: 3251568. DOI: 10.1371/journal.pone.0029416. View

Yoshioka S, King M, Ran S, Okuda H, MacLean 2nd J, McAsey M . WNT7A regulates tumor growth and progression in ovarian cancer through the WNT/β-catenin pathway. Mol Cancer Res. 2012; 10(3):469-82. PMC: 3307825. DOI: 10.1158/1541-7786.MCR-11-0177. View

10.

Staal F, Luis T, Tiemessen M . WNT signalling in the immune system: WNT is spreading its wings. Nat Rev Immunol. 2008; 8(8):581-93. DOI: 10.1038/nri2360. View

11.

Xiong W, Hu L, Jian Y, Wang L, Jiang M, Li W . Wnt5a participates in hepatic stellate cell activation observed by gene expression profile and functional assays. World J Gastroenterol. 2012; 18(15):1745-52. PMC: 3332287. DOI: 10.3748/wjg.v18.i15.1745. View

12.

Wend P, Holland J, Ziebold U, Birchmeier W . Wnt signaling in stem and cancer stem cells. Semin Cell Dev Biol. 2010; 21(8):855-63. DOI: 10.1016/j.semcdb.2010.09.004. View

13.

Reya T, Clevers H . Wnt signalling in stem cells and cancer. Nature. 2005; 434(7035):843-50. DOI: 10.1038/nature03319. View

14.

Brekman A, Singh K, Polotskaia A, Kundu N, Bargonetti J . A p53-independent role of Mdm2 in estrogen-mediated activation of breast cancer cell proliferation. Breast Cancer Res. 2011; 13(1):R3. PMC: 3109566. DOI: 10.1186/bcr2804. View

15.

Watters J, Roberts C . Developing gene expression signatures of pathway deregulation in tumors. Mol Cancer Ther. 2006; 5(10):2444-9. DOI: 10.1158/1535-7163.MCT-06-0340. View

16.

Iravani S, Mora L, Miller F, Dawson P . Altered expression of c-erbB-2, DF3, b72.3, p53 and Ki-67 with progression and differentiation to two distinct histologic types of invasive carcinoma in the MCF10AT human xenograft model of proliferative breast disease. Int J Oncol. 1998; 12(2):369-75. DOI: 10.3892/ijo.12.2.369. View

17.

Huelsken J, Behrens J . The Wnt signalling pathway. J Cell Sci. 2002; 115(Pt 21):3977-8. DOI: 10.1242/jcs.00089. View

18.

Malhotra S, Kincade P . Wnt-related molecules and signaling pathway equilibrium in hematopoiesis. Cell Stem Cell. 2009; 4(1):27-36. PMC: 2975490. DOI: 10.1016/j.stem.2008.12.004. View