Assessment of the Tumourigenic and Metastatic Properties of SK-MEL28 Melanoma Cells Surviving Electrochemotherapy with Bleomycin

Overview

Journal Radiol Oncol

Publisher Sciendo

Specialties Oncology
Radiology

Date 2012 Aug 31

PMID 22933978

Citations 5

Authors

Vesna Todorovic

Gregor Sersa

Vid Mlakar

Damjan Glavac

Maja Cemazar

Affiliations

Soon will be listed here.

Abstract

Background: Electrochemotherapy is a local treatment combining chemotherapy and electroporation and is highly effective treatment approach for subcutaneous tumours of various histologies. Contrary to surgery and radiation, the effect of electrochemotherapy on metastatic potential of tumour cells has not been extensively studied. The aim of the study was to evaluate the effect of electrochemotherapy with bleomycin on the metastatic potential of human melanoma cells in vitro.

Materials And Methods: Viable cells 48 hours after electrochemotherapy were tested for their ability to migrate and invade through Matrigel coated porous membrane. In addition, microarray analysis and quantitative Real-Time PCR were used to detect changes in gene expression after electrochemotherapy.

Results: Cell migration and invasion were not changed in melanoma cells surviving electrochemotherapy. Interestingly, only a low number of tumourigenesis related genes was differentially expressed after electrochemotherapy.

Conclusions: Our data suggest that metastatic potential of human melanoma cells is not affected by electrochemotherapy with bleomycin, confirming safe role of electrochemotherapy in the clinics.

Citing Articles

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Cytosolic DNA Sensor Upregulation Accompanies DNA Electrotransfer in B16.F10 Melanoma Cells.

Znidar K, Bosnjak M, Cemazar M, Heller L Mol Ther Nucleic Acids. 2016; 5(6):e322.

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Modulation of activity of known cytotoxic ruthenium(III) compound (KP418) with hampered transmembrane transport in electrochemotherapy in vitro and in vivo.

Hudej R, Miklavcic D, Cemazar M, Todorovic V, Sersa G, Bergamo A J Membr Biol. 2014; 247(12):1239-51.

PMID: 24957014 DOI: 10.1007/s00232-014-9696-2.

Electrotransfer of single-stranded or double-stranded DNA induces complete regression of palpable B16.F10 mouse melanomas.

Heller L, Todorovic V, Cemazar M Cancer Gene Ther. 2013; 20(12):695-700.

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Staurosporine induces different cell death forms in cultured rat astrocytes.

Simenc J, Lipnik-Stangelj M Radiol Oncol. 2013; 46(4):312-20.

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