Triple Touch Sperm Immobilization Vs. Single Touch Sperm Immobilization in ICSI - a Randomised Trial

Overview

Journal Reprod Biol Endocrinol

Publisher Biomed Central

Specialties Endocrinology
Reproductive Medicine

Date 2012 Aug 30

PMID 22929301

Citations 2

Authors

An Velaers

Goedele Paternot

Sophie Debrock

Thomas DHooghe

Carl Spiessens

Affiliations

Soon will be listed here.

Abstract

Background: Although different techniques for sperm immobilization have been described, their value has not been assessed in an adequately powered randomized study. The aim of this study was to compare two types of sperm immobilization methods prior to ICSI and to test the hypothesis that triple touch immobilization (TTIm) would lead to a higher (5% -65% up to 70%) fertilization rate (FR) than single touch immobilization (STIm).

Methods: A total of 3056 metaphase II (MII) oocytes, from 290 patients, were randomly assigned to the STIm group (n = 1528 oocytes; 145 cycles) or to the TTIm group (n = 1528 oocytes; 138 cycles). A total of 1478 oocytes (STIm group) and 1476 oocytes (TTIm group) were used in the statistical analysis. The primary outcome variable was FR. Secondary outcome variables included: number of good quality embryos (GQE) on day 2 and day 3, implantation rate (IR) and implantation with foetal heart beat rate (FHB). Statistical analysis was done using the Fisher Exact test with a significance level of 0.05.

Results: The results showed no differences in FR between both groups. The proportion of good quality embryos on day 3, was significantly higher in the STIm group (37.5%) compared to the TTIm group (31.8%; p = 0.02).

Conclusions: In this RCT, the hypothesis that the post-ICSI FR would be higher after TTIm than after STIm was not confirmed and the number of good quality embryos on day 3 was significantly lower in the TTIm group than in the STIm group. These data suggest that more 'aggressive' TTIm technique has no advantages compared to the STIm technique.

Citing Articles

Aggressive sperm immobilization improves reproductive outcomes in patients with suboptimal semen parameters and previous ICSI fertilization failure.

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References

Takeuchi T, Colombero L, Neri Q, Rosenwaks Z, Palermo G . Does ICSI require acrosomal disruption? An ultrastructural study. Hum Reprod. 2003; 19(1):114-7. DOI: 10.1093/humrep/deg511. View

van Steirteghem A, Nagy Z, Joris H, Liu J, Staessen C, Smitz J . High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod. 1993; 8(7):1061-6. DOI: 10.1093/oxfordjournals.humrep.a138192. View

Tucker M, MORTON P, Witt M, Wright G . Intracytoplasmic injection of testicular and epididymal spermatozoa for treatment of obstructive azoospermia. Hum Reprod. 1995; 10(3):486-9. DOI: 10.1093/oxfordjournals.humrep.a135969. View

Chen S, Ho H, Chen H, Huang S, Lee T, Yang Y . Intracytoplasmic sperm injection (ICSI) for severe semen abnormalities: dissecting the tail of spermatozoa at the tip. Hum Reprod. 1996; 11(12):2640-4. DOI: 10.1093/oxfordjournals.humrep.a019185. View

Fishel S, Lisi F, Rinaldi L, Green S, Hunter A, Dowell K . Systematic examination of immobilizing spermatozoa before intracytoplasmic sperm injection in the human. Hum Reprod. 1995; 10(3):497-500. DOI: 10.1093/oxfordjournals.humrep.a135974. View

Dozortsev D, Rybouchkin A, De Sutter P, Dhont M . Sperm plasma membrane damage prior to intracytoplasmic sperm injection: a necessary condition for sperm nucleus decondensation. Hum Reprod. 1995; 10(11):2960-4. DOI: 10.1093/oxfordjournals.humrep.a135829. View

Debrock S, Spiessens C, Afschrift H, Van der Auwera I, DHooghe T . Application of the Fertilase-laser system versus the conventional mechanical method to immobilize spermatozoa for intracytoplasmic sperm injection. A randomized controlled trial. Gynecol Obstet Invest. 2003; 56(2):102-5. DOI: 10.1159/000072995. View

Debrock S, Melotte C, Spiessens C, Peeraer K, Vanneste E, Meeuwis L . Preimplantation genetic screening for aneuploidy of embryos after in vitro fertilization in women aged at least 35 years: a prospective randomized trial. Fertil Steril. 2009; 93(2):364-73. DOI: 10.1016/j.fertnstert.2008.10.072. View

Palermo G, Schlegel P, Colombero L, Zaninovic N, Moy F, Rosenwaks Z . Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates. Hum Reprod. 1996; 11(5):1023-9. DOI: 10.1093/oxfordjournals.humrep.a019290. View

10.

Gerris J, Mangelschots K, van Royen E, Joostens M, Eestermans W, RYCKAERT G . ICSI and severe male-factor infertility: breaking the sperm tail prior to injection. Hum Reprod. 1995; 10(3):484-6. DOI: 10.1093/oxfordjournals.humrep.a135968. View

11.

Piomboni P, Focarelli R, Stendardi A, Ferramosca A, Zara V . The role of mitochondria in energy production for human sperm motility. Int J Androl. 2011; 35(2):109-24. DOI: 10.1111/j.1365-2605.2011.01218.x. View

12.

Montag M, Rink K, Delacretaz G, Van der Ven H . Laser-induced immobilization and plasma membrane permeabilization in human spermatozoa. Hum Reprod. 2000; 15(4):846-52. DOI: 10.1093/humrep/15.4.846. View

13.

Palermo G, Joris H, Derde M, Camus M, Devroey P, VAN Steirteghem A . Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril. 1993; 59(4):826-35. DOI: 10.1016/s0015-0282(16)55867-1. View

14.

Hoshi K, Yanagida K, Yazawa H, Katayose H, Sato A . Intracytoplasmic sperm injection using immobilized or motile human spermatozoon. Fertil Steril. 1995; 63(6):1241-5. DOI: 10.1016/s0015-0282(16)57604-3. View

15.

van Steirteghem A, Liu J, Joris H, Nagy Z, Janssenswillen C, Tournaye H . Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod. 1993; 8(7):1055-60. DOI: 10.1093/oxfordjournals.humrep.a138191. View

16.

Palermo G, Cohen J, Alikani M, Adler A, Rosenwaks Z . Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil Steril. 1995; 63(6):1231-40. DOI: 10.1016/s0015-0282(16)57603-1. View

17.

Lee B, Shim S, Kim Y, Kim S . The presence of centrioles and centrosomes in ovarian mature cystic teratoma cells suggests human parthenotes developed in vitro can differentiate into mature cells without a sperm centriole. Biochem Biophys Res Commun. 2011; 415(2):401-4. DOI: 10.1016/j.bbrc.2011.10.081. View

18.

Yanagida K, Katayose H, Yazawa H, Kimura Y, Konnai K, Sato A . The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. Hum Reprod. 1999; 14(2):448-53. DOI: 10.1093/humrep/14.2.448. View

19.

Zegers-Hochschild F, Adamson G, de Mouzon J, Ishihara O, Mansour R, Nygren K . International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil Steril. 2009; 92(5):1520-4. DOI: 10.1016/j.fertnstert.2009.09.009. View

20.

Van den Bergh M, Bertrand E, Biramane J, Englert Y . Importance of breaking a spermatozoon's tail before intracytoplasmic injection: a prospective randomized trial. Hum Reprod. 1995; 10(11):2819-20. DOI: 10.1093/oxfordjournals.humrep.a135798. View