Ultrasensitive CDNA Detection of Dengue Virus RNA Using Electrochemical Nanoporous Membrane-based Biosensor

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Aug 29

PMID 22927927

Citations 20

Authors

Varun Rai

Hapuarachchige C Hapuarachchi

Lee Ching Ng

Siew Hwa Soh

Yee Sin Leo

Chee-Seng Toh

Affiliations

Soon will be listed here.

Abstract

A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5'-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 µm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the pores influences the pore size and ionic conductivity. The biosensor demonstrates linear range over 6 order of magnitude with ultrasensitive detection limit of 9.55×10(-12) M for the quantification of ss-31 mer DNA sequence. Its applicability is challenged against real time cDNA PCR sample of dengue virus serotype1 derived from asymmetric PCR. Excellent specificity down to one nucleotide mismatch in target DNA sample of DENV3 is also demonstrated.

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