In Vitro Infection with Classical Swine Fever Virus Inhibits the Transcription of Immune Response Genes

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2012 Aug 29

PMID 22925563

Citations 5

Authors

Li Feng

Xiao-Quan Li

Xiao-Ning Li

Jun Li

Xian-Ming Meng

Hong-Yun Zhang

Jing-Jing Liang

Hui Li

Shi-Kai Sun

Xin-Bin Cai

Li-Juan Su

Shan Yin

Yan-Sheng Li

Ting Rong Luo

Affiliations

Soon will be listed here.

Abstract

Background: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear.

Results: In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p < 0.001).

Conclusion: We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF.

Citing Articles

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Recombinant Swinepox Virus Expressing Glycoprotein E2 of Classical Swine Fever Virus Confers Complete Protection in Pigs upon Viral Challenge.

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Tang H, Lu Z, Wei X, Zhong T, Zhong Y, Ouyang L Sci Rep. 2016; 6:30529.

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Interaction of CSFV E2 protein with swine host factors as detected by yeast two-hybrid system.

Gladue D, Baker-Bransetter R, Holinka L, Fernandez-Sainz I, ODonnell V, Fletcher P PLoS One. 2014; 9(1):e85324.

PMID: 24416391 PMC: 3885694. DOI: 10.1371/journal.pone.0085324.

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