Comparison of Cell Proliferation and Epigenetic Modification of Gene Expression Patterns in Canine Foetal Fibroblasts and Adipose Tissue-derived Mesenchymal Stem Cells

Overview

Journal Cell Prolif

Date 2012 Aug 29

PMID 22925503

Citations 6

Authors

H J Oh

E J Park

S Y Lee

J W Soh

I S Kong

S W Choi

J C Ra

S K Kang

B C Lee

Affiliations

Soon will be listed here.

Abstract

Objectives: This study compared rate of cell proliferation, viability, cell size, expression patterns of genes related to pluripotency and epigenetic modification between canine foetal fibroblasts (cFF) and canine adipose tissue-derived mesenchymal stem cells (cAd-MSC).

Materials And Methods: Proliferation pattern, cell viability as well as cell size at each passage of cFF and cAd-MSC were measured when cultures reached confluence. In addition, real-time PCR was performed to investigate expression of Dnmt1, HDAC1, OCT4, SOX2, BAX, BCL2 genes with reference to β-actin gene expression as an endogenous control in both cell lines.

Results: cFF and cAd-MSC differed in number of generations, but not in doubling times, at all passages. Mean cell size of cAd-MSC was significantly smaller than that of cFF. Cell viability was significantly lower in cFFs and apoptotic level was significantly lower in cAd-MSC compared to passage-matched cFF. In the expression of genes related to pluripotency and epigenetic modification, level of HDAC1 in cAd-MSC was significantly higher than in cFF, but expression of Dnmt1 did not differ between the two groups. OCT4 and SOX2 were significantly more highly expressed in cAd-MSC compared to cFF.

Conclusions: cAd-MSC have higher stem-cell potential than cFF in terms of proliferation patterns, epigenetic modification and pluripotency, thus cAd-MSC could be more appropriate than cFF as donors of nuclei in somatic cell nuclear transfer for transgenesis.

Citing Articles

Optimal Treatment of 6-Dimethylaminopurine Enhances the In Vivo Development of Canine Embryos by Rapid Initiation of DNA Synthesis.

Oh H, Lee B, Kim M Int J Mol Sci. 2021; 22(14).

PMID: 34299380 PMC: 8303139. DOI: 10.3390/ijms22147757.

Decellularized human amniotic membrane: how viable is it as a delivery system for human adipose tissue-derived stromal cells?.

Gholipourmalekabadi M, Sameni M, Radenkovic D, Mozafari M, Mossahebi-Mohammadi M, Seifalian A Cell Prolif. 2016; 49(1):115-21.

PMID: 26840647 PMC: 6496672. DOI: 10.1111/cpr.12240.

Adipose-tissue and intestinal inflammation - visceral obesity and creeping fat.

Kredel L, Siegmund B Front Immunol. 2014; 5:462.

PMID: 25309544 PMC: 4174117. DOI: 10.3389/fimmu.2014.00462.

Hip osteoarthritis in dogs: a randomized study using mesenchymal stem cells from adipose tissue and plasma rich in growth factors.

Cuervo B, Rubio M, Sopena J, Dominguez J, Vilar J, Morales M Int J Mol Sci. 2014; 15(8):13437-60.

PMID: 25089877 PMC: 4159804. DOI: 10.3390/ijms150813437.

Assessment of the effect of intraarticular injection of autologous adipose-derived mesenchymal stem cells in osteoarthritic dogs using a double blinded force platform analysis.

Vilar J, Batista M, Morales M, Santana A, Cuervo B, Rubio M BMC Vet Res. 2014; 10:143.

PMID: 24984756 PMC: 4085658. DOI: 10.1186/1746-6148-10-143.

References

Kern S, Eichler H, Stoeve J, Kluter H, Bieback K . Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301. DOI: 10.1634/stemcells.2005-0342. View

Schwarz C, Leicht U, Drosse I, Ulrich V, Luibl V, Schieker M . Characterization of adipose-derived equine and canine mesenchymal stem cells after incubation in agarose-hydrogel. Vet Res Commun. 2011; 35(8):487-99. DOI: 10.1007/s11259-011-9492-8. View

Maurisse R, de Semir D, Emamekhoo H, Bedayat B, Abdolmohammadi A, Parsi H . Comparative transfection of DNA into primary and transformed mammalian cells from different lineages. BMC Biotechnol. 2010; 10:9. PMC: 2830169. DOI: 10.1186/1472-6750-10-9. View

Broske A, Vockentanz L, Kharazi S, Huska M, Mancini E, Scheller M . DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction. Nat Genet. 2009; 41(11):1207-15. DOI: 10.1038/ng.463. View

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

Dovey O, Foster C, Cowley S . Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation. Proc Natl Acad Sci U S A. 2010; 107(18):8242-7. PMC: 2889513. DOI: 10.1073/pnas.1000478107. View

Li H, Dai K, Tang T, Zhang X, Yan M, Lou J . Bone regeneration by implantation of adipose-derived stromal cells expressing BMP-2. Biochem Biophys Res Commun. 2007; 356(4):836-42. DOI: 10.1016/j.bbrc.2007.02.165. View

Meissner A, Mikkelsen T, Gu H, Wernig M, Hanna J, Sivachenko A . Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature. 2008; 454(7205):766-70. PMC: 2896277. DOI: 10.1038/nature07107. View

Hong S, Kim M, Jang G, Oh H, Park J, Kang J . Generation of red fluorescent protein transgenic dogs. Genesis. 2009; 47(5):314-22. DOI: 10.1002/dvg.20504. View

10.

Moon J, Kim S, Park H, Kang J, Park S, Koo O . Production of porcine cloned embryos derived from cells conditionally expressing an exogenous gene using Cre-loxP. Zygote. 2012; 20(4):423-5. DOI: 10.1017/S0967199411000773. View

11.

Schaffler A, Buchler C . Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies. Stem Cells. 2007; 25(4):818-27. DOI: 10.1634/stemcells.2006-0589. View

12.

Schnieke A, Kind A, Ritchie W, Mycock K, Scott A, Ritchie M . Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science. 1998; 278(5346):2130-3. DOI: 10.1126/science.278.5346.2130. View

13.

ZuK P, Zhu M, Ashjian P, De Ugarte D, Huang J, Mizuno H . Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13(12):4279-95. PMC: 138633. DOI: 10.1091/mbc.e02-02-0105. View

14.

Yap F, Cheong S, Ammu R, Leong C . Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties. Malays J Pathol. 2010; 31(2):113-20. View

15.

Cibelli J, Stice S, Golueke P, KANE J, Jerry J, Blackwell C . Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science. 1998; 280(5367):1256-8. DOI: 10.1126/science.280.5367.1256. View

16.

Mack G . Cancer researchers usher in dog days of medicine. Nat Med. 2005; 11(10):1018. DOI: 10.1038/nm1005-1018a. View

17.

Smith S . Gilbert's conjecture: the search for DNA (cytosine-5) demethylases and the emergence of new functions for eukaryotic DNA (cytosine-5) methyltransferases. J Mol Biol. 2000; 302(1):1-7. DOI: 10.1006/jmbi.2000.4046. View

18.

Kim M, Oh H, Park J, Kim G, Hong S, Jang G . Generation of transgenic dogs that conditionally express green fluorescent protein. Genesis. 2011; 49(6):472-8. DOI: 10.1002/dvg.20737. View

19.

Chew J, Loh Y, Zhang W, Chen X, Tam W, Yeap L . Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells. Mol Cell Biol. 2005; 25(14):6031-46. PMC: 1168830. DOI: 10.1128/MCB.25.14.6031-6046.2005. View

20.

Zuk P, Zhu M, Mizuno H, Huang J, Futrell J, Katz A . Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001; 7(2):211-28. DOI: 10.1089/107632701300062859. View