Detection of Human Herpes Viruses in Patients with Chronic and Aggressive Periodontitis and Relationship Between Viruses and Clinical Parameters

Overview

Journal J Oral Maxillofac Pathol

Date 2012 Aug 28

PMID 22923891

Citations 27

Authors

Sushma Das

G Shobha Prakash Krithiga

S Gopalakrishnan

Affiliations

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Abstract

Background And Aims: Recent microbiological researches have revealed the possible role of human cytomegalovirus (HCMV), Epstein barr virus (EBV), and herpes simplex virus (HSV-1 and HSV-2) in the etiopathogenesis of periodontal diseases. The present pilot study has been undertaken to detect the presence of these viruses in chronic periodontitis, aggressive periodontitis, and healthy individuals and to determine the relationship between these viruses and the clinical parameters.

Materials And Methods: A total of 10 patients belonging to the age group of 18 to 55 years were included. The patients were randomly assigned into periodontally healthy (sulcus depth ≤ 3 mm), chronic periodontitis, and aggressive periodontitis with pockets measuring ≥6 mm. Seventy-five subgingival plaque samples (25 samples from each group) were collected and subjected to multiplex polymerase chain reaction for the detection of presence of HCMV, EBV, HSV-1, and HSV-2. The results were analyzed using one-way ANOVA for multiple group comparisons followed by Student's t-test for pair-wise comparisons. Categorical data was analyzed by Fisher's exact test.

Results: HSV-1 was detected in 76% (P<0.001) of sites with chronic periodontitis and 80% (P<0.001) sites with aggressive periodontitis. EBV was detected in 32% (P<0.05) of sites with chronic periodontitis and aggressive periodontitis. The probing pocket depth and clinical attachment level was statistically significant in HSV-1 detected sites compared with undetected sites in aggressive periodontitis patients

Conclusion: Among these viruses HSV-1 and EBV were found to be significantly associated with destructive periodontal disease, including chronic and aggressive periodontitis. Further, HSV-1 was found to be associated with severity and progression of destructive periodontal disease.

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References

Contreras A, Slots J . Typing of herpes simplex virus from human periodontium. Oral Microbiol Immunol. 2001; 16(1):63-4. DOI: 10.1034/j.1399-302x.2001.160111.x. View

Contreras A, Umeda M, Chen C, Bakker I, Morrison J, Slots J . Relationship between herpesviruses and adult periodontitis and periodontopathic bacteria. J Periodontol. 1999; 70(5):478-84. DOI: 10.1902/jop.1999.70.5.478. View

Yapar M, Saygun I, Ozdemir A, Kubar A, Sahin S . Prevalence of human herpesviruses in patients with aggressive periodontitis. J Periodontol. 2003; 74(11):1634-40. DOI: 10.1902/jop.2003.74.11.1634. View

SILNESS J, Loe H . PERIODONTAL DISEASE IN PREGNANCY. II. CORRELATION BETWEEN ORAL HYGIENE AND PERIODONTAL CONDTION. Acta Odontol Scand. 1964; 22:121-35. DOI: 10.3109/00016356408993968. View

Kamma J, Slots J . Herpesviral-bacterial interactions in aggressive periodontitis. J Clin Periodontol. 2003; 30(5):420-6. DOI: 10.1034/j.1600-051x.2003.20002.x. View

Ehrlich J, Cohen G, Hochman N . Specific herpes simplex virus antigen in human gingiva. J Periodontol. 1983; 54(6):357-60. DOI: 10.1902/jop.1983.54.6.357. View

Zakay-Rones Z, Hochman N, Rones Y . Immunological response to herpes simplex virus in human gingival fluid. J Periodontol. 1982; 53(1):42-5. DOI: 10.1902/jop.1982.53.1.42. View

Saygun I, Sahin S, Ozdemir A, Kurtis B, Yapar M, Kubar A . Detection of human viruses in patients with chronic periodontitis and the relationship between viruses and clinical parameters. J Periodontol. 2003; 73(12):1437-43. DOI: 10.1902/jop.2002.73.12.1437. View

Kubar A, Saygun I, Yapar M, Ozdemir A, Slots J . Real-time PCR quantification of cytomegalovirus in aggressive periodontitis lesions using TaqMan technology. J Periodontal Res. 2004; 39(2):81-6. DOI: 10.1111/j.1600-0765.2004.00707.x. View

10.

Armitage G . Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000. 2004; 34:9-21. DOI: 10.1046/j.0906-6713.2002.003421.x. View

11.

Markoulatos P, Georgopoulou A, Siafakas N, Plakokefalos E, Tzanakaki G, Kourea-Kremastinou J . Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay. J Clin Microbiol. 2001; 39(12):4426-32. PMC: 88560. DOI: 10.1128/JCM.39.12.4426-4432.2001. View

12.

Saygun I, Kubar A, Ozdemir A, Yapar M, Slots J . Herpesviral-bacterial interrelationships in aggressive periodontitis. J Periodontal Res. 2004; 39(4):207-12. DOI: 10.1111/j.1600-0765.2004.00728.x. View

13.

Ling L, Ho C, Wu C, Chen Y, Hung S . Association between human herpesviruses and the severity of periodontitis. J Periodontol. 2005; 75(11):1479-85. DOI: 10.1902/jop.2004.75.11.1479. View

14.

Loe H, SILNESS J . PERIODONTAL DISEASE IN PREGNANCY. I. PREVALENCE AND SEVERITY. Acta Odontol Scand. 1963; 21:533-51. DOI: 10.3109/00016356309011240. View

15.

Contreras A, Nowzari H, Slots J . Herpesviruses in periodontal pocket and gingival tissue specimens. Oral Microbiol Immunol. 2001; 15(1):15-8. DOI: 10.1034/j.1399-302x.2000.150103.x. View

16.

Amit R, Morag A, Ravid Z, Hochman N, Ehrlich J, Zakay-Rones Z . Detection of herpes simplex virus in gingival tissue. J Periodontol. 1992; 63(6):502-6. DOI: 10.1902/jop.1992.63.6.502. View

17.

Contreras A, Slots J . Mammalian viruses in human periodontitis. Oral Microbiol Immunol. 1996; 11(6):381-6. DOI: 10.1111/j.1399-302x.1996.tb00199.x. View

18.

Konstantinidis A, Sakellari D, Papa A, Antoniadis A . Real-time polymerase chain reaction quantification of Epstein--Barr virus in chronic periodontitis patients. J Periodontal Res. 2005; 40(4):294-8. DOI: 10.1111/j.1600-0765.2005.00796.x. View

19.

Kubar A, Saygun I, Ozdemir A, Yapar M, Slots J . Real-time polymerase chain reaction quantification of human cytomegalovirus and Epstein-Barr virus in periodontal pockets and the adjacent gingiva of periodontitis lesions. J Periodontal Res. 2005; 40(2):97-104. DOI: 10.1111/j.1600-0765.2005.00770.x. View