A Chinese Herbal Formula "Gan-Lu-Yin" Suppresses Vascular Smooth Muscle Cell Migration by Inhibiting Matrix Metalloproteinase-2/9 Through the PI3K/AKT and ERK Signaling Pathways

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2012 Aug 28

PMID 22920190

Citations 7

Authors

Yi-Chung Chien

Ming-Jyh Sheu

Chieh-Hsi Wu

Wen-Hsin Lin

Ying-Yi Chen

Po-Liang Cheng

Hsu-Chen Cheng

Affiliations

Soon will be listed here.

Abstract

Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Citing Articles

Traditional Chinese Medicine in Patients With Primary Sjogren's Syndrome: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial.

Chen H, Lai J, Yu M, Chen C, Hsieh Y, Hsu Y Front Med (Lausanne). 2021; 8:744194.

PMID: 34651000 PMC: 8505713. DOI: 10.3389/fmed.2021.744194.

(Wall.) Seem Improves Intimal Hyperplasia after Vascular Injury by Downregulating the Wnt3/Dvl-1/-Catenin Pathway.

Zhao X, Huang J, Mo Z, Wei J, Zhong C, Teng H Biomed Res Int. 2021; 2021:6682525.

PMID: 34337044 PMC: 8292040. DOI: 10.1155/2021/6682525.

Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis.

Inagaki Y, Kido J, Nishikawa Y, Kido R, Sakamoto E, Bando M J Clin Med. 2021; 10(3).

PMID: 33498415 PMC: 7926381. DOI: 10.3390/jcm10030386.

Anti-inflammatory effect of Ganluyin, a Chinese classic prescription, in chronic pharyngitis rat model.

Chen Y, Luo R, Lei S, Li B, Zhou F, Wang H BMC Complement Med Ther. 2020; 20(1):265.

PMID: 32859182 PMC: 7456022. DOI: 10.1186/s12906-020-03057-5.

Ursodeoxycholic acid inhibits intimal hyperplasia, vascular smooth muscle cell excessive proliferation, migration via blocking miR-21/PTEN/AKT/mTOR signaling pathway.

Huang R, Huang Y, Zeng G, Li M, Jin Y Cell Cycle. 2020; 19(8):918-932.

PMID: 32202193 PMC: 7217369. DOI: 10.1080/15384101.2020.1732514.

References

Meng X, Jin Y, Yu Y, Bai J, Liu G, Zhu J . Characterisation of fibronectin-mediated FAK signalling pathways in lung cancer cell migration and invasion. Br J Cancer. 2009; 101(2):327-34. PMC: 2720209. DOI: 10.1038/sj.bjc.6605154. View

Hsieh Y, Hsieh S, Chang Y, Hsueh C, Hsu S . The lipoxygenase inhibitor, baicalein, modulates cell adhesion and migration by up-regulation of integrins and vinculin in rat heart endothelial cells. Br J Pharmacol. 2007; 151(8):1235-45. PMC: 2189825. DOI: 10.1038/sj.bjp.0707345. View

Kumagai T, Muller C, Desmond J, Imai Y, Heber D, Koeffler H . Scutellaria baicalensis, a herbal medicine: anti-proliferative and apoptotic activity against acute lymphocytic leukemia, lymphoma and myeloma cell lines. Leuk Res. 2006; 31(4):523-30. DOI: 10.1016/j.leukres.2006.08.019. View

Choi K, Kim J, Song N, Son J, Hwang M, Byun S . Phosphoinositide 3-kinase is a novel target of piceatannol for inhibiting PDGF-BB-induced proliferation and migration in human aortic smooth muscle cells. Cardiovasc Res. 2009; 85(4):836-44. DOI: 10.1093/cvr/cvp359. View

Ma Z, Otsuyama K, Liu S, Abroun S, Ishikawa H, Tsuyama N . Baicalein, a component of Scutellaria radix from Huang-Lian-Jie-Du-Tang (HLJDT), leads to suppression of proliferation and induction of apoptosis in human myeloma cells. Blood. 2005; 105(8):3312-8. DOI: 10.1182/blood-2004-10-3915. View

Chao J, Chiang S, Wang C, Tsai Y, Wu M . Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells. World J Gastroenterol. 2006; 12(28):4478-84. PMC: 4125633. DOI: 10.3748/wjg.v12.i28.4478. View

Wu C, Pan J, Chang W, Hung J, Mao S . The molecular mechanism of actinomycin D in preventing neointimal formation in rat carotid arteries after balloon injury. J Biomed Sci. 2005; 12(3):503-12. DOI: 10.1007/s11373-005-6900-5. View

Bauters C, Lablanche J, McFadden E, Leroy F, Bertrand M . Clinical characteristics and angiographic follow-up of patients undergoing early or late repeat dilation for a first restenosis. J Am Coll Cardiol. 1992; 20(4):845-8. DOI: 10.1016/0735-1097(92)90182-m. View

Pan C, Hsieh I, Liu F, Hsieh W, Sheu M, Koizumi A . Effects of a Chinese herbal health formula, "Gan-Lu-Yin", on angiogenesis. J Agric Food Chem. 2010; 58(13):7685-92. DOI: 10.1021/jf1002254. View

10.

Ikezoe T, Chen S, Heber D, Taguchi H, Koeffler H . Baicalin is a major component of PC-SPES which inhibits the proliferation of human cancer cells via apoptosis and cell cycle arrest. Prostate. 2001; 49(4):285-92. DOI: 10.1002/pros.10024. View

11.

Jin U, Lee J, Kang S, Kim J, Park W, Kim J . A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci. 2005; 77(22):2760-9. DOI: 10.1016/j.lfs.2005.02.028. View

12.

Lin I, Lee M, Chuang W . Application of LC/MS and ICP/MS for establishing the fingerprint spectrum of the traditional Chinese medicinal preparation Gan-Lu-Yin. J Sep Sci. 2006; 29(1):172-9. DOI: 10.1002/jssc.200500147. View

13.

Indolfi C, Coppola C, Torella D, Arcucci O, Chiariello M . Gene therapy for restenosis after balloon angioplasty and stenting. Cardiol Rev. 2001; 7(6):324-31. DOI: 10.1097/00045415-199911000-00010. View

14.

Gennaro G, Menard C, Michaud S, deBlois D, Rivard A . Inhibition of vascular smooth muscle cell proliferation and neointimal formation in injured arteries by a novel, oral mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Circulation. 2004; 110(21):3367-71. DOI: 10.1161/01.CIR.0000147773.86866.CD. View

15.

Lai K, Huang A, Hsu S, Kuo C, Yang J, Wu S . Benzyl isothiocyanate (BITC) inhibits migration and invasion of human colon cancer HT29 cells by inhibiting matrix metalloproteinase-2/-9 and urokinase plasminogen (uPA) through PKC and MAPK signaling pathway. J Agric Food Chem. 2010; 58(5):2935-42. DOI: 10.1021/jf9036694. View

16.

Huang G, Yang C, Chang Y, Amagaya S, Wang H, Hou W . Hispolon suppresses SK-Hep1 human hepatoma cell metastasis by inhibiting matrix metalloproteinase-2/9 and urokinase-plasminogen activator through the PI3K/Akt and ERK signaling pathways. J Agric Food Chem. 2010; 58(17):9468-75. DOI: 10.1021/jf101508r. View

17.

Jung J, Kim H, Lee C, Park D, Kim Y, Lee M . Caffeic acid and its synthetic derivative CADPE suppress tumor angiogenesis by blocking STAT3-mediated VEGF expression in human renal carcinoma cells. Carcinogenesis. 2007; 28(8):1780-7. DOI: 10.1093/carcin/bgm130. View

18.

Ross R . Atherosclerosis--an inflammatory disease. N Engl J Med. 1999; 340(2):115-26. DOI: 10.1056/NEJM199901143400207. View

19.

Gruntzig A, SENNING A, SIEGENTHALER W . Nonoperative dilatation of coronary-artery stenosis: percutaneous transluminal coronary angioplasty. N Engl J Med. 1979; 301(2):61-8. DOI: 10.1056/NEJM197907123010201. View

20.

Sheu M, Cheng H, Chien Y, Chou P, Huang G, Chen J . Molecular mechanism of green microalgae, Dunaliella salina, involved in attenuating balloon injury-induced neointimal formation. Br J Nutr. 2010; 104(3):326-35. DOI: 10.1017/S0007114510000693. View