Antimicrobial Activity and Genetic Profile of Enteroccoci Isolated from Hoopoes Uropygial Gland

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Aug 23

PMID 22911858

Citations 16

Authors

Magdalena Ruiz-Rodriguez

Eva Valdivia

Manuel Martin-Vivaldi

Antonio M Martin-Platero

Manuel Martinez-Bueno

Maria Mendez

Juan M Peralta-Sanchez

Juan J Soler

Affiliations

Soon will be listed here.

Abstract

Symbiotic microorganisms may be directly transferred from parents to offspring or acquired from a particular environment that animals may be able to select. If benefits for hosts vary among microbial strains, natural selection may favour hosts holding the most beneficial one. Enterococci symbionts living in the hoopoe (Upupa epops) uropygial gland are able to synthesise bacteriocins (antimicrobial peptides that inhibit the growth of competitor bacteria). We explored variability in genetic profile (through RAPD-PCR analyses) and antimicrobial properties (by performing antagonistic tests against ten bacterial indicator strains) of the different isolates obtained from the uropygial glands of hoopoe females and nestlings. We found that the genetic profile of bacterial isolates was related to antimicrobial activity, as well as to individual host identity and the nest from which samples were obtained. This association suggest that variation in the inhibitory capacity of Enterococci symbionts should be under selection.

Citing Articles

Nesting hoopoes cultivate in their uropygial gland the microbial symbionts with the highest antimicrobial capacity.

Soler J, Baron M, Martinez-Renau E, Zhang L, Liang W, Martin-Vivaldi M Sci Rep. 2024; 14(1):30797.

PMID: 39730533 PMC: 11681103. DOI: 10.1038/s41598-024-81062-1.

Analysis of differential effects of host plants on the gut microbes of .

Zhang B, Yang W, He Q, Chen H, Che B, Bai X Front Microbiol. 2024; 15:1392586.

PMID: 38962140 PMC: 11221597. DOI: 10.3389/fmicb.2024.1392586.

Distinct gut bacterial composition in reared on two host plants.

Wang X, Wang H, Zeng J, Cui Z, Geng S, Song X Front Microbiol. 2023; 14:1199994.

PMID: 37405158 PMC: 10315502. DOI: 10.3389/fmicb.2023.1199994.

Microbial infection risk predicts antimicrobial potential of avian symbionts.

Martinez-Renau E, Mazorra-Alonso M, Ruiz-Castellano C, Martin-Vivaldi M, Martin-Platero A, Baron M Front Microbiol. 2022; 13:1010961.

PMID: 36478864 PMC: 9719979. DOI: 10.3389/fmicb.2022.1010961.

Valorization Potential of a Novel Bacterial Strain, RSP75, towards Lignocellulose Bioconversion: An Assessment of Symbiotic Bacteria from the Stored Grain Pest, .

Dar M, Dhole N, Xie R, Pawar K, Ullah K, Rahi P Microorganisms. 2021; 9(9).

PMID: 34576846 PMC: 8468446. DOI: 10.3390/microorganisms9091952.

References

Abriouel H, Lucas R, Ben Omar N, Valdivia E, Maqueda M, Martinez-Canamero M . Enterocin AS-48RJ: a variant of enterocin AS-48 chromosomally encoded by Enterococcus faecium RJ16 isolated from food. Syst Appl Microbiol. 2005; 28(5):383-97. DOI: 10.1016/j.syapm.2005.01.007. View

Yagi Y, Clewell D . Recombination-deficient mutant of Streptococcus faecalis. J Bacteriol. 1980; 143(2):966-70. PMC: 294401. DOI: 10.1128/jb.143.2.966-970.1980. View

Wolf J, Brodie Iii E, Cheverud J, Moore A, Wade M . Evolutionary consequences of indirect genetic effects. Trends Ecol Evol. 2011; 13(2):64-9. DOI: 10.1016/s0169-5347(97)01233-0. View

Martin-Platero A, Valdivia E, Ruiz-Rodriguez M, Soler J, Martin-Vivaldi M, Maqueda M . Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops). Appl Environ Microbiol. 2006; 72(6):4245-9. PMC: 1489579. DOI: 10.1128/AEM.02940-05. View

Rossetti L, Giraffa G . Rapid identification of dairy lactic acid bacteria by M13-generated, RAPD-PCR fingerprint databases. J Microbiol Methods. 2005; 63(2):135-44. DOI: 10.1016/j.mimet.2005.03.001. View

G Williams J, Kubelik A, Livak K, Rafalski J, Tingey S . DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990; 18(22):6531-5. PMC: 332606. DOI: 10.1093/nar/18.22.6531. View

Ruiz-Rodriguez M, Valdivia E, Soler J, Martin-Vivaldi M, Martin-Platero A, Martinez-Bueno M . Symbiotic bacteria living in the hoopoe's uropygial gland prevent feather degradation. J Exp Biol. 2009; 212(Pt 22):3621-6. DOI: 10.1242/jeb.031336. View

Martin-Platero A, Valdivia E, Maqueda M, Martinez-Bueno M . Fast, convenient, and economical method for isolating genomic DNA from lactic acid bacteria using a modification of the protein "salting-out" procedure. Anal Biochem. 2007; 366(1):102-4. DOI: 10.1016/j.ab.2007.03.010. View

Bisson I, Marra P, Burtt Jr E, Sikaroodi M, Gillevet P . A molecular comparison of plumage and soil bacteria across biogeographic, ecological, and taxonomic scales. Microb Ecol. 2007; 54(1):65-81. DOI: 10.1007/s00248-006-9173-2. View

10.

Banning J, Weddle A, Wahl 3rd G, Simon M, Lauer A, Walters R . Antifungal skin bacteria, embryonic survival, and communal nesting in four-toed salamanders, Hemidactylium scutatum. Oecologia. 2008; 156(2):423-9. DOI: 10.1007/s00442-008-1002-5. View

11.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

12.

Shnit-Orland M, Kushmaro A . Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiol Ecol. 2009; 67(3):371-80. DOI: 10.1111/j.1574-6941.2008.00644.x. View

13.

Gil-Turnes M, Hay M, Fenical W . Symbiotic marine bacteria chemically defend crustacean embryos from a pathogenic fungus. Science. 1989; 246(4926):116-8. DOI: 10.1126/science.2781297. View

14.

Koch A, Kuhn G, Fontanillas P, Fumagalli L, Goudet J, Sanders I . High genetic variability and low local diversity in a population of arbuscular mycorrhizal fungi. Proc Natl Acad Sci U S A. 2004; 101(8):2369-74. PMC: 356957. DOI: 10.1073/pnas.0306441101. View

15.

Riley M, Wertz J . Bacteriocins: evolution, ecology, and application. Annu Rev Microbiol. 2002; 56:117-37. DOI: 10.1146/annurev.micro.56.012302.161024. View

16.

Lindquist N, Barber P, Weisz J . Episymbiotic microbes as food and defence for marine isopods: unique symbioses in a hostile environment. Proc Biol Sci. 2005; 272(1569):1209-16. PMC: 1564109. DOI: 10.1098/rspb.2005.3082. View

17.

Devriese L, Pot B, Collins M . Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol. 1993; 75(5):399-408. DOI: 10.1111/j.1365-2672.1993.tb02794.x. View

18.

Oliver K, Russell J, Moran N, Hunter M . Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Proc Natl Acad Sci U S A. 2003; 100(4):1803-7. PMC: 149914. DOI: 10.1073/pnas.0335320100. View

19.

Gil-Turnes M, Fenical W . Embryos of Homarus americanus are Protected by Epibiotic Bacteria. Biol Bull. 2018; 182(1):105-108. DOI: 10.2307/1542184. View

20.

Gardner A, West S, Buckling A . Bacteriocins, spite and virulence. Proc Biol Sci. 2004; 271(1547):1529-35. PMC: 1691756. DOI: 10.1098/rspb.2004.2756. View