Non-colony Type Monolayer Culture of Human Embryonic Stem Cells

Overview

Journal Stem Cell Res

Publisher Elsevier

Specialty Cell Biology

Date 2012 Aug 23

PMID 22910561

Citations 30

Authors

Kevin G Chen

Barbara S Mallon

Rebecca S Hamilton

Olga A Kozhich

Kyeyoon Park

Daniel J Hoeppner

Pamela G Robey

Ronald D G McKay

Affiliations

Soon will be listed here.

Abstract

Regenerative medicine, relying on human embryonic stem cell (hESC) technology, opens promising new avenues for therapy of many severe diseases. However, this approach is restricted by limited production of the desired cells due to the refractory properties of hESC growth in vitro. It is further hindered by insufficient control of cellular stress, growth rates, and heterogeneous cellular states under current culture conditions. In this study, we report a novel cell culture method based on a non-colony type monolayer (NCM) growth. Human ESCs under NCM remain pluripotent as determined by teratoma assays and sustain the potential to differentiate into three germ layers. This NCM culture has been shown to homogenize cellular states, precisely control growth rates, significantly increase cell production, and enhance hESC recovery from cryopreservation without compromising chromosomal integrity. This culture system is simple, robust, scalable, and suitable for high-throughput screening and drug discovery.

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