Mucus Digestion Improves the Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae on the Cobas 4800

Overview

Journal Sex Transm Dis

Specialty Infectious Diseases

Date 2012 Aug 21

PMID 22902672

Citations 2

Authors

Amanda Miller

Collette Bromhead

Mark Jones

Paul Tustin

Affiliations

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Abstract

We compared the performance of cobas 4800 PCR for Neisseria gonorrhoeae with culture and found an association between pipetting errors and positive culture, caused by mucopurulent discharge. The problem may be resolved by pretreating samples with Sputasol, thus improving detection of both Chlamydia trachomatis and Neisseria gonorrhoeae.

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