Prevalence and Genetic Characterization of Powassan Virus Strains Infecting Ixodes Scapularis in Connecticut

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2012 Aug 15

PMID 22890037

Citations 49

Authors

John F Anderson

Philip M Armstrong

Affiliations

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Abstract

A total of 30 Powassan virus (POWV) isolates from Ixodes scapularis collected from Bridgeport and North Branford, CT in 2008, 2010, 2011, and 2012 and one earlier isolate from Ixodes cookei collected in Old Lyme, CT in 1978 were characterized by phylogenetic analysis of their envelope gene sequences. Powassan virus sequences segregated into two major groups termed the deer tick virus (DTV) and Powassan (POW) lineages. The lineage from I. cookei was POW. The remaining viruses from I. scapularis grouped with the DTV lineage. Powassan viruses from Bridgeport were nearly identical and clustered with a virus strain from a human in New York. Viruses from North Branford were homogeneous and grouped with viruses from Massachusetts, northwestern Connecticut, and Ontario. These findings suggest that POWV was independently introduced into these geographical locations in Connecticut and maintained focally in their respective environments. An improved method of isolation of POWV in vitro is described.

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References

Main A, Carey A, DOWNS W . Powassan virus in Ixodes cookei and Mustelidae in New England. J Wildl Dis. 1979; 15(4):585-91. DOI: 10.7589/0090-3558-15.4.585. View

Ebel G, Foppa I, Spielman A, Telford 2nd S . A focus of deer tick virus transmission in the northcentral United States. Emerg Infect Dis. 1999; 5(4):570-4. PMC: 2627731. DOI: 10.3201/eid0504.990423. View

WALLIS R, Brown S, Kloter K, Main Jr A . Erythema chronicum migrans and lyme arthritis: field study of ticks. Am J Epidemiol. 1978; 108(4):322-7. DOI: 10.1093/oxfordjournals.aje.a112626. View

Ebel G, Spielman A, Telford S . Phylogeny of North American Powassan virus. J Gen Virol. 2001; 82(Pt 7):1657-1665. DOI: 10.1099/0022-1317-82-7-1657. View

Telford 3rd S, Armstrong P, Katavolos P, Foppa I, Garcia A, Wilson M . A new tick-borne encephalitis-like virus infecting New England deer ticks, Ixodes dammini. Emerg Infect Dis. 1997; 3(2):165-70. PMC: 2627606. DOI: 10.3201/eid0302.970209. View

Stafford 3rd K, DeNicola A, Kilpatrick H . Reduced abundance of Ixodes scapularis (Acari: Ixodidae) and the tick parasitoid Ixodiphagus hookeri (Hymenoptera: Encyrtidae) with reduction of white-tailed deer. J Med Entomol. 2003; 40(5):642-52. DOI: 10.1603/0022-2585-40.5.642. View

McLean D, Donohue W . Powassan virus: isolation of virus from a fatal case of encephalitis. Can Med Assoc J. 1959; 80(9):708-11. PMC: 1830849. View

Ebel G, Campbell E, Goethert H, Spielman A, Telford 3rd S . Enzootic transmission of deer tick virus in New England and Wisconsin sites. Am J Trop Med Hyg. 2001; 63(1-2):36-42. DOI: 10.4269/ajtmh.2000.63.36. View

Ebel G . Update on Powassan virus: emergence of a North American tick-borne flavivirus. Annu Rev Entomol. 2009; 55:95-110. DOI: 10.1146/annurev-ento-112408-085446. View

10.

. Outbreak of Powassan encephalitis--Maine and Vermont, 1999-2001. MMWR Morb Mortal Wkly Rep. 2002; 50(35):761-4. View

11.

KARABATSOS N . Supplement to International Catalogue of Arboviruses including certain other viruses of vertebrates. Am J Trop Med Hyg. 1978; 27(2 Pt 2 Suppl):372-440. DOI: 10.4269/ajtmh.1978.27.372. View

12.

Labuda M, Nuttall P, Kozuch O, Eleckova E, Williams T, Zuffova E . Non-viraemic transmission of tick-borne encephalitis virus: a mechanism for arbovirus survival in nature. Experientia. 1993; 49(9):802-5. DOI: 10.1007/BF01923553. View

13.

Williams S, Ward J, Worthley T, Stafford 3rd K . Managing Japanese barberry (Ranunculales: Berberidaceae) infestations reduces blacklegged tick (Acari: Ixodidae) abundance and infection prevalence with Borrelia burgdorferi (Spirochaetales: Spirochaetaceae). Environ Entomol. 2009; 38(4):977-84. DOI: 10.1603/022.038.0404. View

14.

Brackney D, Nofchissey R, Fitzpatrick K, Brown I, Ebel G . Stable prevalence of Powassan virus in Ixodes scapularis in a northern Wisconsin focus. Am J Trop Med Hyg. 2008; 79(6):971-3. PMC: 5467223. View

15.

GOLDFIELD M, Austin S, Black H, Taylor B, Altman R . A non-fatal human case of Powassan virus encephalitis. Am J Trop Med Hyg. 1973; 22(1):78-81. DOI: 10.4269/ajtmh.1973.22.78. View

16.

Anderson J, Magnarelli L . Vertebrate host relationships and distribution of ixodid ticks (Acari: Ixodidae) in Connecticut, USA. J Med Entomol. 1980; 17(4):314-23. DOI: 10.1093/jmedent/17.4.314. View

17.

COSTERO A, Grayson M . Experimental transmission of Powassan virus (Flaviviridae) by Ixodes scapularis ticks (Acari:Ixodidae). Am J Trop Med Hyg. 1996; 55(5):536-46. DOI: 10.4269/ajtmh.1996.55.536. View

18.

Gholam B, Puksa S, Provias J . Powassan encephalitis: a case report with neuropathology and literature review. CMAJ. 2000; 161(11):1419-22. PMC: 1230834. View

19.

Hinten S, Beckett G, Gensheimer K, Pritchard E, Courtney T, Sears S . Increased recognition of Powassan encephalitis in the United States, 1999-2005. Vector Borne Zoonotic Dis. 2008; 8(6):733-40. DOI: 10.1089/vbz.2008.0022. View

20.

Pesko K, Torres-Perez F, Hjelle B, Ebel G . Molecular epidemiology of Powassan virus in North America. J Gen Virol. 2010; 91(Pt 11):2698-705. PMC: 3052558. DOI: 10.1099/vir.0.024232-0. View