Targeting the Glycoproteome

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 2012 Aug 14

PMID 22886069

Citations 18

Authors

Jonas Nilsson

Adnan Halim

Ammi Grahn

Goran Larson

Affiliations

Soon will be listed here.

Abstract

Despite numerous original publications describing the structural complexity of N- and O-linked glycans on glycoproteins, only very few answer the basic question of which particular glycans are linked to which amino acid residues along the polypeptide chain. Such structural information is of fundamental importance for understanding the biological roles of complex glycosylations as well as deciphering their non-template driven biosynthesis. This review focuses on presenting and commenting on recent strategies, specifically aimed at identifying the glycoproteome of cultured cells and biological samples, using targeted and global enrichment procedures and utilizing the high resolution power, high through-put capacity and complementary fragmentation techniques of tandem mass spectrometry. The goal is to give an update of this emerging field of protein and glyco-sciences and suggest routes to bridge the data gap between the two aspects of glycoprotein characteristics, i.e. glycan structures and their attachment sites.

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References

Tabak L . The role of mucin-type O-glycans in eukaryotic development. Semin Cell Dev Biol. 2010; 21(6):616-21. PMC: 2902666. DOI: 10.1016/j.semcdb.2010.02.001. View

Gerken T, Jamison O, Perrine C, Collette J, Moinova H, Ravi L . Emerging paradigms for the initiation of mucin-type protein O-glycosylation by the polypeptide GalNAc transferase family of glycosyltransferases. J Biol Chem. 2011; 286(16):14493-507. PMC: 3077648. DOI: 10.1074/jbc.M111.218701. View

Scott N, Parker B, Connolly A, Paulech J, Edwards A, Crossett B . Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of.... Mol Cell Proteomics. 2010; 10(2):M000031-MCP201. PMC: 3033663. DOI: 10.1074/mcp.M000031-MCP201. View

Laine R . Glycoconjugates: overview and strategy. Methods Enzymol. 1990; 193:539-53. DOI: 10.1016/0076-6879(90)93437-p. View

Sihlbom C, Davidsson P, Nilsson C . Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry. J Proteome Res. 2005; 4(6):2294-301. DOI: 10.1021/pr050210g. View

Tian E, Ten Hagen K . Recent insights into the biological roles of mucin-type O-glycosylation. Glycoconj J. 2008; 26(3):325-34. PMC: 2656418. DOI: 10.1007/s10719-008-9162-4. View

Nagata Y, Burger M . Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding. J Biol Chem. 1974; 249(10):3116-22. View

Lei Z, Beuerman R, Chew A, Koh S, Cafaro T, Urrets-Zavalia E . Quantitative analysis of N-linked glycoproteins in tear fluid of climatic droplet keratopathy by glycopeptide capture and iTRAQ. J Proteome Res. 2009; 8(4):1992-2003. DOI: 10.1021/pr800962q. View

Zougman A, Pilch B, Podtelejnikov A, Kiehntopf M, Schnabel C, Kumar C . Integrated analysis of the cerebrospinal fluid peptidome and proteome. J Proteome Res. 2007; 7(1):386-99. DOI: 10.1021/pr070501k. View

10.

Marth J, Grewal P . Mammalian glycosylation in immunity. Nat Rev Immunol. 2008; 8(11):874-87. PMC: 2768770. DOI: 10.1038/nri2417. View

11.

Ten Hagen K, Fritz T, Tabak L . All in the family: the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases. Glycobiology. 2003; 13(1):1R-16R. DOI: 10.1093/glycob/cwg007. View

12.

Nilsson J, Nilsson J, Larson G, Grahn A . Characterization of site-specific O-glycan structures within the mucin-like domain of alpha-dystroglycan from human skeletal muscle. Glycobiology. 2010; 20(9):1160-9. DOI: 10.1093/glycob/cwq082. View

13.

Alley Jr W, Mechref Y, Novotny M . Characterization of glycopeptides by combining collision-induced dissociation and electron-transfer dissociation mass spectrometry data. Rapid Commun Mass Spectrom. 2008; 23(1):161-70. DOI: 10.1002/rcm.3850. View

14.

Hagglund P, Matthiesen R, Elortza F, Hojrup P, Roepstorff P, Jensen O . An enzymatic deglycosylation scheme enabling identification of core fucosylated N-glycans and O-glycosylation site mapping of human plasma proteins. J Proteome Res. 2007; 6(8):3021-31. DOI: 10.1021/pr0700605. View

15.

Brown K, Vanderver A, Hoffman E, Schiffmann R, Hathout Y . Characterization of Transferrin Glycopeptide Structures in Human Cerebrospinal Fluid. Int J Mass Spectrom. 2012; 312:97-106. PMC: 3293479. DOI: 10.1016/j.ijms.2011.06.021. View

16.

Harazono A, Kawasaki N, Kawanishi T, Hayakawa T . Site-specific glycosylation analysis of human apolipoprotein B100 using LC/ESI MS/MS. Glycobiology. 2004; 15(5):447-62. DOI: 10.1093/glycob/cwi033. View

17.

Zhang H, Li X, Martin D, Aebersold R . Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nat Biotechnol. 2003; 21(6):660-6. DOI: 10.1038/nbt827. View

18.

Wells L, Vosseller K, Hart G . Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc. Science. 2001; 291(5512):2376-8. DOI: 10.1126/science.1058714. View

19.

Larsen M, Hojrup P, Roepstorff P . Characterization of gel-separated glycoproteins using two-step proteolytic digestion combined with sequential microcolumns and mass spectrometry. Mol Cell Proteomics. 2004; 4(2):107-19. DOI: 10.1074/mcp.M400068-MCP200. View

20.

Steentoft C, Vakhrushev S, Vester-Christensen M, Schjoldager K, Kong Y, Bennett E . Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines. Nat Methods. 2011; 8(11):977-82. DOI: 10.1038/nmeth.1731. View