The Rice Wall-associated Receptor-like Kinase Gene OsDEES1 Plays a Role in Female Gametophyte Development

Overview

Journal Plant Physiol

Specialty Physiology

Date 2012 Aug 14

PMID 22885936

Citations 38

Authors

Na Wang

Hui-Jia Huang

Su-Ting Ren

Jiao-Jiao Li

Ying Sun

Da-Ye Sun

Su-Qiao Zhang

Affiliations

Soon will be listed here.

Abstract

The wall-associated kinase (WAK) gene family is a unique subfamily of receptor-like kinases (RLKs) in plants. WAK-RLKs play roles in cell expansion, pathogen resistance, and metal tolerance in Arabidopsis (Arabidopsis thaliana). Rice (Oryza sativa) has far more WAK-RLK genes than Arabidopsis, but the functions of rice WAK-RLKs are poorly understood. In this study, we found that one rice WAK-RLK gene, DEFECT IN EARLY EMBRYO SAC1 (OsDEES1), is involved in the regulation of early embryo sac development. OsDEES1 silencing by RNA interference caused a high rate of female sterility. Crossing experiments showed that female reproductive organs lacking OsDEES1 carried a functional defect. A detailed investigation of the ovaries from OsDEES1 RNA interference plants indicated that the knockdown of OsDEES1 expression did not affect megasporogenesis but that it disturbed female gametophyte formation, resulting in a degenerated embryo sac and defective seed formation. OsDEES1 exhibited a tissue-specific expression pattern in flowers and seedlings. In the ovary, OsDEES1 was expressed in the megagametophyte region and surrounding nucellus cells in the ovule near the micropylar region. OsDEES1 was found to be a membrane-localized protein with a unique sequence compared with other WAK-RLKs. These data indicate that OsDEES1 plays a role in rice sexual reproduction by regulating female gametophyte development. This study offers new insight into the functions of the WAK-RLK family.

Citing Articles

Function of WAKs in Regulating Cell Wall Development and Responses to Abiotic Stress.

Yao X, Humphries J, Johnson K, Chen J, Ma Y Plants (Basel). 2025; 14(3).

PMID: 39942905 PMC: 11820136. DOI: 10.3390/plants14030343.

Genome-wide identification of the wall-associated kinase gene family and their expression patterns under various abiotic stresses in soybean ( (L.) Merr).

Li X, Qi S, Meng L, Su P, Sun Y, Li N Front Plant Sci. 2025; 15:1511681.

PMID: 39886685 PMC: 11779729. DOI: 10.3389/fpls.2024.1511681.

Characterization of the wall-associated kinase (WAK) gene family in Gossypium barbadense reveals the positive role of GbWAK5 in salt tolerance.

Zhang Z, Ma W, Wang H, Ren Z, Liu Y, He K Plant Cell Rep. 2024; 44(1):18.

PMID: 39738693 DOI: 10.1007/s00299-024-03407-4.

Lectin Receptor-Like Protein Kinase OsNRFG6 is Required for Embryo Sac Development and Fertilization in Neo-Tetraploid Rice.

Zhao C, Li Q, Ge Q, Chen R, Yu H, Wu J Rice (N Y). 2024; 17(1):41.

PMID: 38916708 PMC: 11199475. DOI: 10.1186/s12284-024-00720-0.

OsRH52A, a DEAD-box protein, regulates functional megaspore specification and is required for embryo sac development in rice.

Huang J, Qiao Z, Yu H, Lu Z, Chen W, Lu J J Exp Bot. 2024; 75(16):4802-4821.

PMID: 38642102 PMC: 11350083. DOI: 10.1093/jxb/erae180.

References

Nonomura K, Miyoshi K, Eiguchi M, Suzuki T, Miyao A, Hirochika H . The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice. Plant Cell. 2003; 15(8):1728-39. PMC: 167165. DOI: 10.1105/tpc.012401. View

Brutus A, Sicilia F, Macone A, Cervone F, De Lorenzo G . A domain swap approach reveals a role of the plant wall-associated kinase 1 (WAK1) as a receptor of oligogalacturonides. Proc Natl Acad Sci U S A. 2010; 107(20):9452-7. PMC: 2889104. DOI: 10.1073/pnas.1000675107. View

Decreux A, Messiaen J . Wall-associated kinase WAK1 interacts with cell wall pectins in a calcium-induced conformation. Plant Cell Physiol. 2005; 46(2):268-78. DOI: 10.1093/pcp/pci026. View

Verica J, He Z . The cell wall-associated kinase (WAK) and WAK-like kinase gene family. Plant Physiol. 2002; 129(2):455-9. PMC: 1540232. DOI: 10.1104/pp.011028. View

Jun L, Saiki R, Tatsumi K, Nakagawa T, Kawamukai M . Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana. Plant Cell Physiol. 2005; 45(12):1882-8. DOI: 10.1093/pcp/pch211. View

Zhao D, Speal B, Ma H . The excess microsporocytes1 gene encodes a putative leucine-rich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther. Genes Dev. 2002; 16(15):2021-31. PMC: 186413. DOI: 10.1101/gad.997902. View

Canales C, Bhatt A, Scott R, Dickinson H . EXS, a putative LRR receptor kinase, regulates male germline cell number and tapetal identity and promotes seed development in Arabidopsis. Curr Biol. 2002; 12(20):1718-27. DOI: 10.1016/s0960-9822(02)01151-x. View

Meier S, Ruzvidzo O, Morse M, Donaldson L, Kwezi L, Gehring C . The Arabidopsis wall associated kinase-like 10 gene encodes a functional guanylyl cyclase and is co-expressed with pathogen defense related genes. PLoS One. 2010; 5(1):e8904. PMC: 2811198. DOI: 10.1371/journal.pone.0008904. View

Yang W, Sundaresan V . Genetics of gametophyte biogenesis in Arabidopsis. Curr Opin Plant Biol. 2000; 3(1):53-7. DOI: 10.1016/s1369-5266(99)00037-0. View

10.

Acosta-Garcia G, Vielle-Calzada J . A classical arabinogalactan protein is essential for the initiation of female gametogenesis in Arabidopsis. Plant Cell. 2004; 16(10):2614-28. PMC: 520959. DOI: 10.1105/tpc.104.024588. View

11.

Chhun T, Aya K, Asano K, Yamamoto E, Morinaka Y, Watanabe M . Gibberellin regulates pollen viability and pollen tube growth in rice. Plant Cell. 2007; 19(12):3876-88. PMC: 2217639. DOI: 10.1105/tpc.107.054759. View

12.

Shiu S, Karlowski W, Pan R, Tzeng Y, Mayer K, Li W . Comparative analysis of the receptor-like kinase family in Arabidopsis and rice. Plant Cell. 2004; 16(5):1220-34. PMC: 423211. DOI: 10.1105/tpc.020834. View

13.

He Z, Cheeseman I, He D, Kohorn B . A cluster of five cell wall-associated receptor kinase genes, Wak1-5, are expressed in specific organs of Arabidopsis. Plant Mol Biol. 1999; 39(6):1189-96. DOI: 10.1023/a:1006197318246. View

14.

Lehti-Shiu M, Zou C, Hanada K, Shiu S . Evolutionary history and stress regulation of plant receptor-like kinase/pelle genes. Plant Physiol. 2009; 150(1):12-26. PMC: 2675737. DOI: 10.1104/pp.108.134353. View

15.

Anderson C, Wagner T, Perret M, He Z, He D, Kohorn B . WAKs: cell wall-associated kinases linking the cytoplasm to the extracellular matrix. Plant Mol Biol. 2001; 47(1-2):197-206. View

16.

Zhang S, Chen C, Li L, Meng L, Singh J, Jiang N . Evolutionary expansion, gene structure, and expression of the rice wall-associated kinase gene family. Plant Physiol. 2005; 139(3):1107-24. PMC: 1283751. DOI: 10.1104/pp.105.069005. View

17.

Hou X, Tong H, Selby J, Dewitt J, Peng X, He Z . Involvement of a cell wall-associated kinase, WAKL4, in Arabidopsis mineral responses. Plant Physiol. 2005; 139(4):1704-16. PMC: 1310553. DOI: 10.1104/pp.105.066910. View

18.

Ling Y, Zhang C, Chen T, Hao H, Liu P, Bressan R . Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis. PLoS One. 2012; 7(1):e29470. PMC: 3253799. DOI: 10.1371/journal.pone.0029470. View

19.

He Z, Fujiki M, Kohorn B . A cell wall-associated, receptor-like protein kinase. J Biol Chem. 1996; 271(33):19789-93. DOI: 10.1074/jbc.271.33.19789. View

20.

Pagnussat G, Alandete-Saez M, Bowman J, Sundaresan V . Auxin-dependent patterning and gamete specification in the Arabidopsis female gametophyte. Science. 2009; 324(5935):1684-9. DOI: 10.1126/science.1167324. View