Comparative Transcriptomics of H. Pylori Strains AM5, SS1 and Their HpyAVIBM Deletion Mutants: Possible Roles of Cytosine Methylation

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Aug 11

PMID 22879937

Citations 23

Authors

Ritesh Kumar

Asish K Mukhopadhyay

Prachetash Ghosh

Desirazu N Rao

Affiliations

Soon will be listed here.

Abstract

Helicobacter pylori is an important human pathogen and one of the most successful chronic colonizers of the human body. H. pylori uses diverse mechanisms to modulate its interaction with the host in order to promote chronic infection and overcome host immune response. Restriction-modification genes are a major part of strain-specific genes present in H. pylori. The role of N(6)--adenine methylation in bacterial gene regulation and virulence is well established but not much is known about the effect of C(5) -cytosine methylation on gene expression in prokaryotes. In this study, it was observed by microarray analysis and RT-PCR, that deletion of an orphan C(5) -cytosine methyltransferase, hpyAVIBM in H. pylori strains AM5and SS1 has a significant effect on the expression of number of genes belonging to motility, adhesion and virulence. AM5ΔhpyAVIBM mutant strain has a different LPS profile and is able to induce high IL-8 production compared to wild-type. hpyAVIBM from strain 26695 is able to complement mutant SS1 and AM5 strains. This study highlights a possible significance of cytosine methylation in the physiology of H. pylori.

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