Effects of Transfer of Embryos Independently Cultured in Essential and Sequential Culture Media on Pregnancy Rates in Assisted Reproduction Cycles

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2012 Aug 7

PMID 22865212

Citations 2

Authors

Selmo Geber

Renata Bossi

Fernando Guimaraes

Marcello Valle

Marcos Sampaio

Affiliations

Soon will be listed here.

Abstract

Purpose: Several culture media are available to be used in ART. However it is uncertain whether embryos would preferably benefit from one type of medium or the association of different media.

Methods: We performed this study to evaluate the impact of simultaneous transfer of embryos independently cultured in two distinct culture media, on pregnancy outcome. A total of 722 couples who underwent infertility treatment were sequentially allocated into three groups: those who had half of the embryos individually cultured in MEM and the other half cultured in sequential media (MEM + Seq Group) (n = 243); those who had all embryos cultured only in sequential medium (Seq Group) (n = 239); and those who had all embryos cultured only in MEM (MEM Group) (n = 240).

Results: The pregnancy rate was higher in the MEM + Seq group (51.8 %) than the Seq group (36.7 %) (p < 0.001). However the pregnancy rate observed in the MEM group was similar to the others (44.2 %). When a logistic regression test was applied it demonstrated that the number of transferred embryos did not interfere in the pregnancy rates.

Conclusions: Our results suggests that offering different culture conditions for sibling embryos with subsequent transfer of embryos that were kept in distinct culture media, might increase pregnancy rates in assisted reproduction cycles.

Citing Articles

Comparison of two commercial embryo culture media (SAGE-1 step single medium vs. G1-PLUSTM/G2-PLUSTM sequential media): Influence on in vitro fertilization outcomes and human embryo quality.

Lopez-Pelayo I, Gutierrez-Romero J, Mangano Armada A, Calero-Ruiz M, Acevedo-Yague P JBRA Assist Reprod. 2018; 22(2):128-133.

PMID: 29697926 PMC: 5982559. DOI: 10.5935/1518-0557.20180024.

Culture media for human pre-implantation embryos in assisted reproductive technology cycles.

Youssef M, Mantikou E, van Wely M, van der Veen F, Al-Inany H, Repping S Cochrane Database Syst Rev. 2015; (11):CD007876.

PMID: 26585317 PMC: 10657458. DOI: 10.1002/14651858.CD007876.pub2.

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