Single-step Reconstitution of Apo-hemoproteins at the Disruption Stage of Escherichia Coli Cells

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2012 Aug 2

PMID 22851307

Citations 10

Authors

Norifumi Kawakami

Osami Shoji

Yoshihito Watanabe

Affiliations

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Abstract

Hemoproteins on their metal: We report a novel strategy for the reconstitution of hemoproteins with non-natural metal complexes; simple addition of manganese and ruthenium porphyrin to E. coli cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins.

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