A Two-component Regulatory System Controls Autoregulated Serpin Expression in Bifidobacterium Breve UCC2003

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2012 Jul 31

PMID 22843530

Citations 20

Authors

Pablo Alvarez-Martin

Mary OConnell Motherway

Francesca Turroni

Elena Foroni

Marco Ventura

Douwe Van Sinderen

Affiliations

Soon will be listed here.

Abstract

This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), which are predicted to encode a hypothetical membrane-associated protein and a serpin-like protein, respectively. The response regulator SerR was shown to bind to the promoter region of ser(2003), and the probable recognition sequence of SerR was determined by a combinatorial approach of in vitro site-directed mutagenesis coupled to transcriptional fusion and electrophoretic mobility shift assays (EMSAs). The importance of the serRK 2CRS in the response of B. breve to protease-mediated induction was confirmed by generating a B. breve serR insertion mutant, which was shown to exhibit altered ser(2003) transcriptional induction patterns compared to the parent strain, UCC2003. Interestingly, the analysis of a B. breve serU mutant revealed that the SerRK signaling pathway appears to include a SerU-dependent autoregulatory loop.

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