Comparison of Three Typing Methods for Pseudomonas Aeruginosa Isolates from Patients with Cystic Fibrosis

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2012 Jul 31

PMID 22843295

Citations 26

Authors

V Waters

J E A Zlosnik

Y C W Yau

D P Speert

S D Aaron

D S Guttman

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to compare two traditional pattern matching techniques, pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD), with the more reproducible technique of multilocus sequence typing (MLST) to genotype a blinded sample of Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients. A blinded sample of 48 well-characterized CF P. aeruginosa isolates was genotyped by PFGE, RAPD, and MLST, each performed in a different laboratory. The discriminatory power and congruence between the methods were compared using the Simpson's index, Rand index, and Wallace coefficient. PFGE and MLST had the greatest congruence with the highest Rand index (0.697). The discriminatory power of PFGE, RAPD, and MLST were comparable, with high Simpson's indices (range 0.973-0.980). MLST identified the most clonal relationships. When clonality was defined as agreement between two or more methods, MLST had the greatest predictive value (100 %) in labeling strains as unique, while PFGE had the greatest predictive value (96 %) in labeling strains as clonal. This study demonstrated the highest level of agreement between PFGE and MLST in genotyping P. aeruginosa isolates from CF patients. MLST had the greatest predictive value in identifying strains as unique and, thus, has the potential to be a cost-efficient, high-throughput, first-pass typing method.

Citing Articles

Genetic Diversity in Antimicrobial Resistance Determinants Among Pathogenic Pseudomonas aeruginosa in India.

Rathod M, Shukla S, Sanapala P, Rajni E, Maheshwari G, Gajjar D Curr Microbiol. 2025; 82(5):189.

PMID: 40080202 DOI: 10.1007/s00284-025-04174-5.

Molecular characterization of the (M)-carrying transposon Tn and plasmids in isolates recovered from swine.

Liu Y, Qiao Z, Ma Y, Wang M, Hu G, Li E Front Vet Sci. 2024; 11:1430398.

PMID: 39507219 PMC: 11539080. DOI: 10.3389/fvets.2024.1430398.

Recombinase-aided amplification assay for rapid detection of imipenem-resistant and rifampin-resistant .

Zhou Y, Shi R, Mu L, Tian L, Zhou M, Lyu W Front Cell Infect Microbiol. 2024; 14:1428827.

PMID: 39318475 PMC: 11420161. DOI: 10.3389/fcimb.2024.1428827.

The airway microbiome of persons with cystic fibrosis correlates with acquisition and microbiological outcomes of incident infection.

Bowron L, Acosta N, Thornton C, Carpentero J, Waddell B, Bharadwaj L Front Microbiol. 2024; 15:1353145.

PMID: 38690371 PMC: 11059027. DOI: 10.3389/fmicb.2024.1353145.

Antimicrobial resistance of : navigating clinical impacts, current resistance trends, and innovations in breaking therapies.

Elfadadny A, Ragab R, Alharbi M, Badshah F, Ibanez-Arancibia E, Farag A Front Microbiol. 2024; 15:1374466.

PMID: 38646632 PMC: 11026690. DOI: 10.3389/fmicb.2024.1374466.

References

Denton M, Kerr K, Mooney L, Keer V, Rajgopal A, Brownlee K . Transmission of colistin-resistant Pseudomonas aeruginosa between patients attending a pediatric cystic fibrosis center. Pediatr Pulmonol. 2002; 34(4):257-61. DOI: 10.1002/ppul.10166. View

White J, Gilbert J, Hill G, Hill E, Huse S, Weightman A . Culture-independent analysis of bacterial fuel contamination provides insight into the level of concordance with the standard industry practice of aerobic cultivation. Appl Environ Microbiol. 2011; 77(13):4527-38. PMC: 3127687. DOI: 10.1128/AEM.02317-10. View

Krogh Johansen H, Moskowitz S, Ciofu O, Pressler T, Hoiby N . Spread of colistin resistant non-mucoid Pseudomonas aeruginosa among chronically infected Danish cystic fibrosis patients. J Cyst Fibros. 2008; 7(5):391-7. DOI: 10.1016/j.jcf.2008.02.003. View

Curran B, Jonas D, Grundmann H, Pitt T, Dowson C . Development of a multilocus sequence typing scheme for the opportunistic pathogen Pseudomonas aeruginosa. J Clin Microbiol. 2004; 42(12):5644-9. PMC: 535286. DOI: 10.1128/JCM.42.12.5644-5649.2004. View

Fothergill J, White J, Foweraker J, Walshaw M, Ledson M, Mahenthiralingam E . Impact of Pseudomonas aeruginosa genomic instability on the application of typing methods for chronic cystic fibrosis infections. J Clin Microbiol. 2010; 48(6):2053-9. PMC: 2884492. DOI: 10.1128/JCM.00019-10. View

OCarroll M, Syrmis M, Wainwright C, Greer R, Mitchell P, Coulter C . Clonal strains of Pseudomonas aeruginosa in paediatric and adult cystic fibrosis units. Eur Respir J. 2004; 24(1):101-6. DOI: 10.1183/09031936.04.00122903. View

Pedersen S, Koch C, Hoiby N, ROSENDAL K . An epidemic spread of multiresistant Pseudomonas aeruginosa in a cystic fibrosis centre. J Antimicrob Chemother. 1986; 17(4):505-16. DOI: 10.1093/jac/17.4.505. View

Kidd T, Grimwood K, Ramsay K, Rainey P, Bell S . Comparison of three molecular techniques for typing Pseudomonas aeruginosa isolates in sputum samples from patients with cystic fibrosis. J Clin Microbiol. 2010; 49(1):263-8. PMC: 3020435. DOI: 10.1128/JCM.01421-10. View

Gibson R, Burns J, Ramsey B . Pathophysiology and management of pulmonary infections in cystic fibrosis. Am J Respir Crit Care Med. 2003; 168(8):918-51. DOI: 10.1164/rccm.200304-505SO. View

10.

Jones A, Govan J, Doherty C, Dodd M, Isalska B, Stanbridge T . Spread of a multiresistant strain of Pseudomonas aeruginosa in an adult cystic fibrosis clinic. Lancet. 2001; 358(9281):557-8. DOI: 10.1016/s0140-6736(01)05714-2. View

11.

Bradbury R, Champion A, Reid D . Poor clinical outcomes associated with a multi-drug resistant clonal strain of Pseudomonas aeruginosa in the Tasmanian cystic fibrosis population. Respirology. 2008; 13(6):886-92. DOI: 10.1111/j.1440-1843.2008.01383.x. View

12.

Speert D . Molecular epidemiology of Pseudomonas aeruginosa. Front Biosci. 2002; 7:e354-61. DOI: 10.2741/A929. View

13.

Grundmann H, Hori S, Tanner G . Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms. J Clin Microbiol. 2001; 39(11):4190-2. PMC: 88515. DOI: 10.1128/JCM.39.11.4190-4192.2001. View

14.

Singh A, Goering R, Simjee S, Foley S, Zervos M . Application of molecular techniques to the study of hospital infection. Clin Microbiol Rev. 2006; 19(3):512-30. PMC: 1539107. DOI: 10.1128/CMR.00025-05. View

15.

Saiman L, Siegel J . Infection control recommendations for patients with cystic fibrosis: Microbiology, important pathogens, and infection control practices to prevent patient-to-patient transmission. Am J Infect Control. 2003; 31(3 Suppl):S1-62. View

16.

Aaron S, Vandemheen K, Ramotar K, Giesbrecht-Lewis T, Tullis E, Freitag A . Infection with transmissible strains of Pseudomonas aeruginosa and clinical outcomes in adults with cystic fibrosis. JAMA. 2010; 304(19):2145-53. DOI: 10.1001/jama.2010.1665. View

17.

Severiano A, Pinto F, Ramirez M, Carrico J . Adjusted Wallace coefficient as a measure of congruence between typing methods. J Clin Microbiol. 2011; 49(11):3997-4000. PMC: 3209087. DOI: 10.1128/JCM.00624-11. View

18.

Al-Aloul M, Crawley J, Winstanley C, Hart C, Ledson M, Walshaw M . Increased morbidity associated with chronic infection by an epidemic Pseudomonas aeruginosa strain in CF patients. Thorax. 2004; 59(4):334-6. PMC: 1763796. DOI: 10.1136/thx.2003.014258. View

19.

Lewis D, Jones A, Parkhill J, Speert D, Govan J, LiPuma J . Identification of DNA markers for a transmissible Pseudomonas aeruginosa cystic fibrosis strain. Am J Respir Cell Mol Biol. 2005; 33(1):56-64. DOI: 10.1165/rcmb.2004-0352OC. View

20.

Fothergill J, Upton A, Pitt T, Hart C, Winstanley C . Diagnostic multiplex PCR assay for the identification of the Liverpool, Midlands 1 and Manchester CF epidemic strains of Pseudomonas aeruginosa. J Cyst Fibros. 2007; 7(3):258-61. DOI: 10.1016/j.jcf.2007.09.002. View