GABA and Glutamate Are Not Colocalized in Mossy Fiber Terminals of Developing Rodent Hippocampus

Overview

Journal Brain Res

Specialty Neurology

Date 2012 Jul 31

PMID 22842523

Citations 9

Authors

Guoxiang Xiong

Lei Zhang

Jelena Mojsilovic-Petrovic

Edguardo Arroyo

Jaclynn Elkind

Suhali Kundu

Brian Johnson

Colin J Smith

Noam A Cohen

Sean M Grady

Akiva S Cohen

Affiliations

Soon will be listed here.

Abstract

It has been hypothesized that, in the developing rodent hippocampus, mossy fiber terminals release GABA together with glutamate. Here, we used transgenic glutamic acid decarboxylase-67 (GAD67)-GFP expressing mice and multi-label immunohistochemistry to address whether glutamatergic and GABAergic markers are colocalized. We demonstrate that in the dentate gyrus, interneurons positive for GABA/GAD are sparsely distributed along the edge of the hilus, in a different pattern from that of the densely packed granule cells. Co-staining for synaptophysin and vesicular glutamate transporter1 (VGLUT1) in postnatal day 14 brain sections from both mice and rats showed mossy fiber terminals as a group of large (2-5 μm in diameter) VGLUT1-positive excitatory presynaptic terminals in the stratum lucidum of area CA3a/b. Furthermore, co-staining for synaptophysin and vesicular GABA transporter (VGAT) revealed a group of small-sized (∼0.5 μm in diameter) inhibitory presynaptic terminals in the same area where identified mossy fiber terminals were present. The two types of terminals appeared to be mutually exclusive, and showed no colocalization. Thus, our results do not support the hypothesis that GABA is released as a neurotransmitter from mossy fiber terminals during development.

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