Recent Advances in the Development of New Transgenic Animal Technology

Overview

Journal Cell Mol Life Sci

Publisher Springer

Specialty Biology

Date 2012 Jul 27

PMID 22833168

Citations 14

Authors

Xiangyang Miao

Affiliations

Soon will be listed here.

Abstract

Transgenic animal technology is one of the fastest growing biotechnology areas. It is used to integrate exogenous genes into the animal genome by genetic engineering technology so that these genes can be inherited and expressed by offspring. The transgenic efficiency and precise control of gene expression are the key limiting factors in the production of transgenic animals. A variety of transgenic technologies are available. Each has its own advantages and disadvantages and needs further study because of unresolved technical and safety issues. Further studies will allow transgenic technology to explore gene function, animal genetic improvement, bioreactors, animal disease models, and organ transplantation. This article reviews the recently developed animal transgenic technologies, including the germ line stem cell-mediated method to improve efficiency, gene targeting to improve accuracy, RNA interference-mediated gene silencing technology, zinc-finger nuclease gene targeting technology and induced pluripotent stem cell technology. These new transgenic techniques can provide a better platform to develop transgenic animals for breeding new animal varieties and promote the development of medical sciences, livestock production, and other fields.

Citing Articles

Cryopreservation Strategies for Poultry Semen: A Comprehensive Review of Techniques and Applications.

Arif A, Zahoor N, Tang J, Tang M, Dong L, Khan S Vet Sci. 2025; 12(2).

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Post-genomic era in agriculture and veterinary science: successful and proposed application of genetic targeting technologies.

Mazloum A, Karagyaur M, Chernyshev R, Schalkwyk A, Jun M, Qiang F Front Vet Sci. 2023; 10:1180621.

PMID: 37601766 PMC: 10434572. DOI: 10.3389/fvets.2023.1180621.

Constitutive and conditional gene knockout mice for the study of intervertebral disc degeneration: Current status, decision considerations, and future possibilities.

Lu Z, Chen P, Xu Q, Li B, Jiang S, Jiang L JOR Spine. 2023; 6(1):e1242.

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Establishment of an Efficient Immortalization Strategy Using HMEJ-Based b Insertion for Bovine Cells.

Zhang Z, Han Z, Guo Y, Liu X, Gao Y, Zhang Y Int J Mol Sci. 2021; 22(22).

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An Event-Specific Real-Time PCR Method for Measuring Transgenic Lysozyme Goat Content in Trace Samples.

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