Enhanced Killing Effect of Nanosecond Pulse Electric Fields on PANC1 and Jurkat Cell Lines in the Presence of Tween 80

Overview

Journal J Membr Biol

Date 2012 Jul 24

PMID 22821216

Citations 1

Authors

Gaurav Basu

Bhargava Subhash Kalluri

Ahmet Can Sabuncu

Christopher J Osgood

Michael W Stacey

Affiliations

Soon will be listed here.

Abstract

We investigated the effects of nanosecond pulse electric fields (nsPEFs) on Jurkat and PANC1 cells, which are human carcinoma cell lines, in the presence of Tween 80 (T80) at a concentration of 0.18% and demonstarted an enhanced killing effect. We used two biological assays to determine cell viability after exposing cells to nsPEFs in the presence of T80 and observed a significant increase in the killing effect of nsPEFs. We did not see a toxic effect of T80 when cells were exposed to surfactant alone. However, we saw a synergistic effect when cells exposed to T80 were combined with the nsPEFs. Increasing the time of exposure for up to 8 h in T80 led to a significant decrease in cell viability when nsPEFs were applied to cells compared to control cells. We also observed cell type-specific swelling in the presence of T80. We suggest that T80 acts as an adjuvant in facilitating the effects of nsPEFs on the cell membrane; however, the limitations of the viability assays were addressed. We conclude that T80 may increase the fragility of the cell membrane, which makes it more susceptible to nsPEF-mediated killing.

Citing Articles

Nanosecond pulsed electric fields (nsPEFs) regulate phenotypes of chondrocytes through Wnt/β-catenin signaling pathway.

Zhang K, Guo J, Ge Z, Zhang J Sci Rep. 2014; 4:5836.

PMID: 25060711 PMC: 5376156. DOI: 10.1038/srep05836.

References

Riehm H, Biedler J . Potentiation of drug effect by Tween 80 in Chinese hamster cells resistant to actinomycin D and daunomycin. Cancer Res. 1972; 32(6):1195-200. View

Chitnis M, Menon R, Gude R . Effect of Tween 80 on adriamycin cytotoxicity in murine P388 leukemia. Tumori. 1984; 70(4):313-5. DOI: 10.1177/030089168407000403. View

Jones M . Surfactants in membrane solubilisation. Int J Pharm. 1999; 177(2):137-59. DOI: 10.1016/s0378-5173(98)00345-7. View

Sehested M, Jensen P, Skovsgaard T, Bindslev N, Demant E, Friche E . Inhibition of vincristine binding to plasma membrane vesicles from daunorubicin-resistant Ehrlich ascites cells by multidrug resistance modulators. Br J Cancer. 1989; 60(6):809-14. PMC: 2247270. DOI: 10.1038/bjc.1989.371. View

Kolb J, Kono S, Schoenbach K . Nanosecond pulsed electric field generators for the study of subcellular effects. Bioelectromagnetics. 2005; 27(3):172-87. DOI: 10.1002/bem.20185. View

Bowman A, Nesin O, Pakhomova O, Pakhomov A . Analysis of plasma membrane integrity by fluorescent detection of Tl(+) uptake. J Membr Biol. 2010; 236(1):15-26. PMC: 2922847. DOI: 10.1007/s00232-010-9269-y. View

Beebe S, White J, Blackmore P, Deng Y, Somers K, Schoenbach K . Diverse effects of nanosecond pulsed electric fields on cells and tissues. DNA Cell Biol. 2003; 22(12):785-96. DOI: 10.1089/104454903322624993. View

Basrur V, Chitnis M, Menon R . Differential agglutination of P388 adriamycin-sensitive and P388 adriamycin-resistant leukemia cells. Oncology. 1983; 40(3):202-4. DOI: 10.1159/000225726. View

Beebe S, Fox P, Rec L, Willis E, Schoenbach K . Nanosecond, high-intensity pulsed electric fields induce apoptosis in human cells. FASEB J. 2003; 17(11):1493-5. DOI: 10.1096/fj.02-0859fje. View

10.

Tsujino I, Yamazaki T, Masutani M, Sawada U, Horie T . Effect of Tween-80 on cell killing by etoposide in human lung adenocarcinoma cells. Cancer Chemother Pharmacol. 1999; 43(1):29-34. DOI: 10.1007/s002800050859. View

11.

Parris C, Masters J, Walker M, Newman B, Riddle P, English P . Intravesical chemotherapy: combination with Tween 80 increases cytotoxicity in vitro. Urol Res. 1987; 15(1):17-20. DOI: 10.1007/BF00256329. View

12.

Chen X, Swanson R, Kolb J, Nuccitelli R, Schoenbach K . Histopathology of normal skin and melanomas after nanosecond pulsed electric field treatment. Melanoma Res. 2009; 19(6):361-71. PMC: 3137734. DOI: 10.1097/CMR.0b013e32832f1558. View

13.

Coors E, Seybold H, Merk H, Mahler V . Polysorbate 80 in medical products and nonimmunologic anaphylactoid reactions. Ann Allergy Asthma Immunol. 2006; 95(6):593-9. DOI: 10.1016/S1081-1206(10)61024-1. View

14.

Zheng Z, Obbard J . Evaluation of an elevated non-ionic surfactant critical micelle concentration in a soil/aqueous system. Water Res. 2002; 36(10):2667-72. DOI: 10.1016/s0043-1354(01)00472-9. View

15.

Peskin A, Winterbourn C . A microtiter plate assay for superoxide dismutase using a water-soluble tetrazolium salt (WST-1). Clin Chim Acta. 2000; 293(1-2):157-66. DOI: 10.1016/s0009-8981(99)00246-6. View

16.

Stacey M, Fox P, Buescher S, Kolb J . Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survival. Bioelectrochemistry. 2011; 82(2):131-4. DOI: 10.1016/j.bioelechem.2011.06.002. View

17.

Kanduser M, Sentjurc M, Miklavcic D . Cell membrane fluidity related to electroporation and resealing. Eur Biophys J. 2005; 35(3):196-204. DOI: 10.1007/s00249-005-0021-y. View

18.

Nuccitelli R, Pliquett U, Chen X, Ford W, Swanson R, Beebe S . Nanosecond pulsed electric fields cause melanomas to self-destruct. Biochem Biophys Res Commun. 2006; 343(2):351-60. PMC: 1513546. DOI: 10.1016/j.bbrc.2006.02.181. View

19.

Vernier P, Sun Y, Marcu L, Salemi S, Craft C, Gundersen M . Calcium bursts induced by nanosecond electric pulses. Biochem Biophys Res Commun. 2003; 310(2):286-95. DOI: 10.1016/j.bbrc.2003.08.140. View

20.

Pakhomov A, Kolb J, White J, Joshi R, Xiao S, Schoenbach K . Long-lasting plasma membrane permeabilization in mammalian cells by nanosecond pulsed electric field (nsPEF). Bioelectromagnetics. 2007; 28(8):655-63. DOI: 10.1002/bem.20354. View