From Single Molecule Fluctuations to Muscle Contraction: a Brownian Model of A.F. Huxley's Hypotheses

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Jul 21

PMID 22815722

Citations 9

Authors

Lorenzo Marcucci

Toshio Yanagida

Affiliations

Soon will be listed here.

Abstract

Muscular force generation in response to external stimuli is the result of thermally fluctuating, cyclical interactions between myosin and actin, which together form the actomyosin complex. Normally, these fluctuations are modelled using transition rate functions that are based on muscle fiber behaviour, in a phenomenological fashion. However, such a basis reduces the predictive power of these models. As an alternative, we propose a model which uses direct single molecule observations of actomyosin fluctuations reported in the literature. We precisely estimate the actomyosin potential bias and use diffusion theory to obtain a brownian ratchet model that reproduces the complete cross-bridge cycle. The model is validated by simulating several macroscopic experimental conditions, while its interpretation is compatible with two different force-generating scenarios.

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