Microdialysis Sampling for Determination of Plasma Protein Binding of Drugs

Overview

Journal Pharm Res

Specialties Pharmacology
Pharmacy

Date 1990 Oct 1

PMID 2281040

Citations 21

Authors

A M Herrera

D O Scott

C E Lunte

Affiliations

Soon will be listed here.

Abstract

The use of microdialysis sampling to study the binding of drugs to plasma proteins was evaluated. Microdialysis sampling is accomplished by placing a short length of dialysis fiber in the sample and perfusing the fiber with a vehicle. Small molecules in the sample, such as drugs, diffuse into the fiber and are transported to collection vials for analysis. Larger molecules, such as proteins and protein-bound drugs are excluded by the dialysis membrane. Microdialysis was found to give values for in vitro protein binding in plasma equivalent to those determined by ultrafiltration. Microdialysis offers advantages in terms of maintaining equilibria and experimental versatility. Microdialysis sampling also provides potential use for in vivo determinations of protein binding.

Citing Articles

Exposure of Infants to Isoniazid via Breast Milk After Maternal Drug Intake of Recommended Doses Is Clinically Insignificant Irrespective of Metaboliser Status. A Physiologically-Based Pharmacokinetic (PBPK) Modelling Approach to Estimate Drug....

Garessus E, Mielke H, Gundert-Remy U Front Pharmacol. 2019; 10:5.

PMID: 30723406 PMC: 6349757. DOI: 10.3389/fphar.2019.00005.

Microdialysis as an Important Technique in Systems Pharmacology-a Historical and Methodological Review.

Hammarlund-Udenaes M AAPS J. 2017; 19(5):1294-1303.

PMID: 28762127 DOI: 10.1208/s12248-017-0108-2.

A Physiologically Based Pharmacokinetic Model of Isoniazid and Its Application in Individualizing Tuberculosis Chemotherapy.

Cordes H, Thiel C, Aschmann H, Baier V, Blank L, Kuepfer L Antimicrob Agents Chemother. 2016; 60(10):6134-45.

PMID: 27480867 PMC: 5038291. DOI: 10.1128/AAC.00508-16.

Pharmacokinetics and pharmacodynamics of clofazimine in a mouse model of tuberculosis.

Swanson R, Adamson J, Moodley C, Ngcobo B, Ammerman N, Dorasamy A Antimicrob Agents Chemother. 2015; 59(6):3042-51.

PMID: 25753644 PMC: 4432183. DOI: 10.1128/AAC.00260-15.

The study on the entrapment efficiency and in vitro release of puerarin submicron emulsion.

Yue P, Lu X, Zhang Z, Yuan H, Zhu W, Zheng Q AAPS PharmSciTech. 2009; 10(2):376-83.

PMID: 19381837 PMC: 2690779. DOI: 10.1208/s12249-009-9216-3.

References

Kurz H, Trunk H, Weitz B . Evaluation of methods to determine protein-binding of drugs. Equilibrium dialysis, ultrafiltration, ultracentrifugation, gel filtration. Arzneimittelforschung. 1977; 27(7):1373-80. View

Yacobi A, Udall J, Levy G . Serum protein binding as a determinant of warfarin body clearance and anticoagulant effect. Clin Pharmacol Ther. 1976; 19(5 Pt 1):552-8. DOI: 10.1002/cpt1976195part1552. View

Sophianopoulos J, Jerkunica I, Lee C, SGOUTAS D . An improved ultrafiltration method for free thyroxine and triiodothyronine in serum. Clin Chem. 1980; 26(1):159-62. View

Singhvi S, Heald A, GADEBUSCH H, RESNICK M, DiFazio L, Leitz M . Human serum protein binding of cephalosporin antibiotics in vitro. J Lab Clin Med. 1977; 89(2):414-20. View

Koup J, Lau A, Brodsky B, Slaughter R . Chloramphenicol pharmacokinetics in hospitalized patients. Antimicrob Agents Chemother. 1979; 15(5):651-7. PMC: 352732. DOI: 10.1128/AAC.15.5.651. View

Boxenbaum H, Berkersky I, Mattaliano V, Kaplan S . Plasma and salivary concentrations of isoniazid in man: preliminary findings in two slow acetylator subjects. J Pharmacokinet Biopharm. 1975; 3(6):443-56. DOI: 10.1007/BF01059476. View

Bachmann K . Rapid determination of the concentration of unbound warfarin in human plasma. Res Commun Chem Pathol Pharmacol. 1974; 9(2):379-82. View

Whitlam J, Brown K . Ultrafiltration in serum protein binding determinations. J Pharm Sci. 1981; 70(2):146-50. DOI: 10.1002/jps.2600700208. View

Scott D, Sorensen L, Lunte C . In vivo microdialysis sampling coupled to liquid chromatography for the study of acetaminophen metabolism. J Chromatogr. 1990; 506:461-9. DOI: 10.1016/s0021-9673(01)91600-0. View

10.

Gazzard B, Ford-Hutchinson A, Smith M, Williams R . The binding of paracetamol to plasma proteins of man and pig. J Pharm Pharmacol. 1973; 25(12):964-7. DOI: 10.1111/j.2042-7158.1973.tb09987.x. View

11.

Meffin P, Winkle R, Peters F, Harrison D . Acebutolol disposition after intravenous administration. Clin Pharmacol Ther. 1977; 22(5 Pt 1):557-67. DOI: 10.1002/cpt1977225part1557. View

12.

Hooper W, Bochner F, Eadie M, Tyrer J . Plasma protein binding of diphenylhydantoin. Effects of sex hormones, renal and hepatic disease. Clin Pharmacol Ther. 1974; 15(3):276-82. DOI: 10.1002/cpt1974153276. View

13.

Shah V, Wallace S, RIEGELMAN S . Microultrafiltration technique for drug-protein binding determination in plasma. J Pharm Sci. 1974; 63(9):1364-7. DOI: 10.1002/jps.2600630905. View

14.

Yacobi A, Udall J, Levy G . Intrasubject variation of warfarin binding to protein in serum of patients with cardiovascular disease. Clin Pharmacol Ther. 1976; 20(3):300-3. DOI: 10.1002/cpt1976203300. View

15.

Aranda J, Sitar D, Parsons W, Loughnan P, NEIMS A . Pharmacokinetic aspects of theophylline in premature newborns. N Engl J Med. 1976; 295(8):413-6. DOI: 10.1056/NEJM197608192950803. View

16.

Sophianopoulos J, Durham S, SOPHIANOPOULOS A, Ragsdale H, Cropper Jr W . Ultrafiltration is theoretically equivalent to equilibrium dialysis but much simpler to carry out. Arch Biochem Biophys. 1978; 187(1):132-7. DOI: 10.1016/0003-9861(78)90015-2. View

17.

Scott D, Bell M, Lunte C . Microdialysis-perfusion sampling for the investigation of phenol metabolism. J Pharm Biomed Anal. 1989; 7(11):1249-59. DOI: 10.1016/0731-7085(89)80128-1. View

18.

Yacobi A, Lampman T, Levy G . Frequency distribution of free warfarin and free phenytoin fraction values in serum of healthy human adults. Clin Pharmacol Ther. 1977; 21(3):283-6. DOI: 10.1002/cpt1977213283. View