Effects of Hydroxypropyl-β-cyclodextrin on the Growth and Morphology of Absidia Coerulea

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2012 Jul 19

PMID 22806198

Authors

Lanyu Cui

Yanbing Shen

Xiaodong Guo

Yizhong Wang

Yu Zheng

Jianmei Luo

Min Wang

Affiliations

Soon will be listed here.

Abstract

Cyclodextrin has been found to be an attractive novel solubilizer due to its unique material properties. Absidia coerulea is widely used in steroid bioconversion. The effects of hydroxypropyl-β-cyclodextrin (HP-β-CD) on the growth, morphology, and steroid-converting activity of A. coerulea CICC 40302 were systematically studied. HP-β-CD affected A. coerulea growth, resulting in changes in its spore morphology and mycelial morphology. It induced an increase in the spore germination rate and a decrease in cell biomass at the stationary phase. Optical microscopy revealed that HP-β-CD altered the mycelial morphology and reduced the pellet compactness of A. coerulea. A convenient and feasible computing method was used to measure pellet compactness, and it demonstrated that the compactness degree of the pellet decreased as HP-β-CD increased, which could be attributed to the modification of the physical properties of the fermentation medium. Moreover, the changing of mycelial morphology influenced steroid-converting activity. The results showed that HP-β-CD had multiple concentration-dependent effects on A. coerulea cells. HP-β-CD in the proper concentration range holds great potential as a biocompatible solubilizer.

References

Manosroi A, Saowakhon S, Manosroi J . Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-beta-cyclodextrin complexation technique. J Steroid Biochem Mol Biol. 2007; 108(1-2):132-6. DOI: 10.1016/j.jsbmb.2007.05.032. View

Elander R . Industrial production of beta-lactam antibiotics. Appl Microbiol Biotechnol. 2003; 61(5-6):385-92. DOI: 10.1007/s00253-003-1274-y. View

Treskatis S, Orgeldinger V, Wolf H, Gilles E . Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition. Biotechnol Bioeng. 1997; 53(2):191-201. DOI: 10.1002/(SICI)1097-0290(19970120)53:2<191::AID-BIT9>3.0.CO;2-J. View

Nanguy S, Perrier-Cornet J, Bensoussan M, Dantigny P . Impact of water activity of diverse media on spore germination of Aspergillus and Penicillium species. Int J Food Microbiol. 2010; 142(1-2):273-6. DOI: 10.1016/j.ijfoodmicro.2010.06.031. View

Shen Y, Wang M, Zhang L, Ma Y, Ma B, Zheng Y . Effects of hydroxypropyl-β-cyclodextrin on cell growth, activity, and integrity of steroid-transforming Arthrobacter simplex and Mycobacterium sp. Appl Microbiol Biotechnol. 2011; 90(6):1995-2003. DOI: 10.1007/s00253-011-3214-6. View

Durante M, Caretto S, Quarta A, De Paolis A, Nisi R, Mita G . β-Cyclodextrins enhance artemisinin production in Artemisia annua suspension cell cultures. Appl Microbiol Biotechnol. 2011; 90(6):1905-13. DOI: 10.1007/s00253-011-3232-4. View

Nair R, Raina S, Keshavarz T, Kerrigan M . Application of fluorescent indicators to analyse intracellular calcium and morphology in filamentous fungi. Fungal Biol. 2011; 115(4-5):326-34. DOI: 10.1016/j.funbio.2010.12.012. View

Justen P, Paul G, Nienow A, Thomas C . Dependence of mycelial morphology on impeller type and agitation intensity. Biotechnol Bioeng. 1996; 52(6):672-84. DOI: 10.1002/(SICI)1097-0290(19961220)52:6<672::AID-BIT5>3.0.CO;2-L. View

Olsvik E, Tucker K, Thomas C, Kristiansen B . Correlation of Aspergillus niger broth rheological properties with biomass concentration and the shape of mycelial aggregates. Biotechnol Bioeng. 1993; 42(9):1046-52. DOI: 10.1002/bit.260420905. View

10.

Sasaki J, Mikami A, Mizoue K, Omura S . Transformation of 25- and 1 alpha-hydroxyvitamin D3 to 1 alpha, 25-dihydroxyvitamin D3 by using Streptomyces sp. strains. Appl Environ Microbiol. 1991; 57(10):2841-6. PMC: 183884. DOI: 10.1128/aem.57.10.2841-2846.1991. View

11.

Paul G, Thomas C . A structured model for hyphal differentiation and penicillin production using Penicillium chrysogenum. Biotechnol Bioeng. 1996; 51(5):558-72. DOI: 10.1002/(SICI)1097-0290(19960905)51:5<558::AID-BIT8>3.0.CO;2-B. View

12.

Bentley M, Vianna R, Wilson S, Collett J . Characterization of the influence of some cyclodextrins on the stratum corneum from the hairless mouse. J Pharm Pharmacol. 1997; 49(4):397-402. DOI: 10.1111/j.2042-7158.1997.tb06813.x. View

13.

Lai T, Li B, Qin G, Tian S . Oxidative damage involves in the inhibitory effect of nitric oxide on spore germination of Penicillium expansum. Curr Microbiol. 2010; 62(1):229-34. DOI: 10.1007/s00284-010-9695-1. View

14.

Judet D, Bensoussan M, Perrier-Cornet J, Dantigny P . Distributions of the growth rate of the germ tubes and germination time of Penicillium chrysogenum conidia depend on water activity. Food Microbiol. 2008; 25(7):902-7. DOI: 10.1016/j.fm.2008.05.007. View

15.

Kim Y, Song H . Effect of fungal pellet morphology on enzyme activities involved in phthalate degradation. J Microbiol. 2009; 47(4):420-4. DOI: 10.1007/s12275-009-0051-8. View