Development of Bone Marrow Mesenchymal Stem Cell Culture in Vitro

Overview

Journal Chin Med J (Engl)

Specialty General Medicine

Date 2012 Jul 18

PMID 22800837

Citations 6

Authors

Li Zhang

Li-Pan Peng

Nan Wu

Le-Ping Li

Affiliations

Soon will be listed here.

Abstract

Objective: To review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC).

Data Sources: The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture".

Study Selection: Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D.

Results: Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems.

Conclusions: Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.

Citing Articles

Impaired autophagy activity-induced abnormal differentiation of bone marrow stem cells is related to adolescent idiopathic scoliosis osteopenia.

Zhang H, Yang G, Li J, Xiao L, Guo C, Wang Y Chin Med J (Engl). 2023; 136(17):2077-2085.

PMID: 36728938 PMC: 10476821. DOI: 10.1097/CM9.0000000000002165.

Ex vivo evaluation of intravitreal mesenchymal stromal cell viability using bioluminescence imaging.

Vilela C, Souza L, Siqueira R, Calado R, Covas D, Paula J Stem Cell Res Ther. 2018; 9(1):155.

PMID: 29895334 PMC: 5998578. DOI: 10.1186/s13287-018-0909-y.

Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering.

Fernandes G, Yang S Bone Res. 2016; 4():16036.

PMID: 28018706 PMC: 5153571. DOI: 10.1038/boneres.2016.36.

Irradiation alters the differentiation potential of bone marrow mesenchymal stem cells.

Wang Y, Zhu G, Wang J, Chen J Mol Med Rep. 2015; 13(1):213-23.

PMID: 26572960 PMC: 4686093. DOI: 10.3892/mmr.2015.4539.

DNA Methylation Changes during In Vitro Propagation of Human Mesenchymal Stem Cells: Implications for Their Genomic Stability?.

Bentivegna A, Miloso M, Riva G, Foudah D, Butta V, Dalpra L Stem Cells Int. 2013; 2013:192425.

PMID: 24288545 PMC: 3833027. DOI: 10.1155/2013/192425.