Cytomolecular Characterization of De Novo Formed Rye B Chromosome Variants

Overview

Journal Mol Cytogenet

Publisher Biomed Central

Specialty Biochemistry

Date 2012 Jul 18

PMID 22800712

Citations 11

Authors

Andre Marques

Sonja Klemme

Marcelo Guerra

Andreas Houben

Affiliations

Soon will be listed here.

Abstract

Unlabelled:

Background: B chromosomes (Bs) are dispensable elements which occur in many species including rye (Secale cereale). We determined the organization of B variants to obtain insights into the origin of B polymorphisms in rye.

Results: The observed B variants were classified according to their morphology and in situ hybridization patterns with the B-specific repeats D1100 and CL11 into (I) long arm iso B, (II) D1100-deficient B and (III) small metacentric B variants. Long arm iso Bs are likely products of a meiotic centromere misdivision and subsequent duplication of the long arm, whereas small B variants are probably generated by chromosome breakage. Some deficient Bs experienced extensive amplification of CL11 repeats.

Conclusions: Both the pericentromere and the nondisjunction control region seem to be involved in the generation of rye B chromosome variants. However, due to the loss of the B-specific nondisjuction control region most of the variants generated are not capable to accumulate in a population.

Citing Articles

Comprehending the dynamism of B chromosomes in their journey towards becoming unselfish.

Rajpal V, Sharma S, Sehgal D, Sharma P, Wadhwa N, Dhakate P Front Cell Dev Biol. 2023; 10:1072716.

PMID: 36684438 PMC: 9846793. DOI: 10.3389/fcell.2022.1072716.

Out of patterns, the euchromatic B chromosome of the grasshopper Abracris flavolineata is not enriched in high-copy repeats.

Milani D, Ruiz-Ruano F, Camacho J, Cabral-de-Mello D Heredity (Edinb). 2021; 127(5):475-483.

PMID: 34482369 PMC: 8551250. DOI: 10.1038/s41437-021-00470-5.

Satellite DNAs Unveil Clues about the Ancestry and Composition of B Chromosomes in Three Grasshopper Species.

Milani D, Bardella V, Ferretti A, Palacios-Gimenez O, Melo A, Moura R Genes (Basel). 2018; 9(11).

PMID: 30373193 PMC: 6265867. DOI: 10.3390/genes9110523.

Evolution of Plant B Chromosome Enriched Sequences.

Marques A, Klemme S, Houben A Genes (Basel). 2018; 9(10).

PMID: 30360448 PMC: 6210368. DOI: 10.3390/genes9100515.

How Next-Generation Sequencing Has Aided Our Understanding of the Sequence Composition and Origin of B Chromosomes.

Ruban A, Schmutzer T, Scholz U, Houben A Genes (Basel). 2017; 8(11).

PMID: 29068386 PMC: 5704207. DOI: 10.3390/genes8110294.

References

Ma L, Vu G, Schubert V, Watanabe K, Stein N, Houben A . Synteny between Brachypodium distachyon and Hordeum vulgare as revealed by FISH. Chromosome Res. 2010; 18(7):841-50. DOI: 10.1007/s10577-010-9166-3. View

Lia V, Confalonieri V, Poggio L . B chromosome polymorphism in maize landraces: adaptive vs. demographic hypothesis of clinal variation. Genetics. 2007; 177(2):895-904. PMC: 2034652. DOI: 10.1534/genetics.107.075440. View

Munoz-Pajares A, Martinez-Rodriguez L, Teruel M, Cabrero J, Camacho J, Perfectti F . A single, recent origin of the accessory B chromosome of the grasshopper Eyprepocnemis plorans. Genetics. 2011; 187(3):853-63. PMC: 3063678. DOI: 10.1534/genetics.110.122713. View

Wintersberger E . DNA amplification: new insights into its mechanism. Chromosoma. 1994; 103(2):73-81. DOI: 10.1007/BF00352315. View

Blunden R, Wilkes T, Forster J, Jimenez M, Sandery M, Karp A . Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences. Genome. 1993; 36(4):706-11. DOI: 10.1139/g93-095. View

Santos J, Jimenez M, Diez M . Synaptic patterns of rye B chromosomes. IV. The B isochromosomes. Heredity (Edinb). 1995; 74 ( Pt 1):100-7. DOI: 10.1038/hdy.1995.12. View

Jones R . B chromosomes in plants. New Phytol. 2021; 131(4):411-434. DOI: 10.1111/j.1469-8137.1995.tb03079.x. View

Bakkali M, Camacho J . The B chromosome polymorphism of the grasshopper Eyprepocnemis plorans in North Africa: III. mutation rate of B chromosomes. Heredity (Edinb). 2004; 92(5):428-33. DOI: 10.1038/sj.hdy.6800437. View

Jones R, Viegas W, Houben A . A century of B chromosomes in plants: so what?. Ann Bot. 2007; 101(6):767-75. PMC: 2710218. DOI: 10.1093/aob/mcm167. View

10.

Puertas M . Nature and evolution of B chromosomes in plants: A non-coding but information-rich part of plant genomes. Cytogenet Genome Res. 2002; 96(1-4):198-205. DOI: 10.1159/000063047. View

11.

Endo T, Nasuda S, Jones N, Dou Q, Akahori A, Wakimoto M . Dissection of rye B chromosomes, and nondisjunction properties of the dissected segments in a common wheat background. Genes Genet Syst. 2008; 83(1):23-30. DOI: 10.1266/ggs.83.23. View

12.

Wilkes T, Francki M, Langridge P, Karp A, Jones R, Forster J . Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH). Chromosome Res. 1995; 3(8):466-72. DOI: 10.1007/BF00713960. View

13.

Houben A, Kynast R, Heim U, Hermann H, Jones R, Forster J . Molecular cytogenetic characterisation of the terminal heterochromatic segment of the B-chromosome of rye (Secale cereale). Chromosoma. 1996; 105(2):97-103. DOI: 10.1007/BF02509519. View