Targeted Tissue Proteomic Analysis of Human Astrocytomas

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2012 Jul 17

PMID 22794670

Citations 10

Authors

Xueping Fang

Chenchen Wang

Brian M Balgley

Kejia Zhao

Weijie Wang

Fang He

Robert J Weil

Cheng S Lee

Affiliations

Soon will be listed here.

Abstract

Complicating proteomic analysis of whole tissues is the obvious problem of cell heterogeneity in tissues, which often results in misleading or confusing molecular findings. Thus, the coupling of tissue microdissection for tumor cell enrichment with capillary isotachophoresis-based selective analyte concentration not only serves as a synergistic strategy to characterize low abundance proteins, but it can also be employed to conduct comparative proteomic studies of human astrocytomas. A set of fresh frozen brain biopsies were selectively microdissected to provide an enriched, high quality, and reproducible sample of tumor cells. Despite sharing many common proteins, there are significant differences in the protein expression level among different grades of astrocytomas. A large number of proteins, such as plasma membrane proteins EGFR and Erbb2, are up-regulated in glioblastoma. Besides facilitating the prioritization of follow-on biomarker selection and validation, comparative proteomics involving measurements in changes of pathways are expected to reveal the molecular relationships among different pathological grades of gliomas and potential molecular mechanisms that drive gliomagenesis.

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