Regulation of Retinal Proteome by Topical Antiglaucomatous Eye Drops in an Inherited Glaucoma Rat Model

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Jul 14

PMID 22792152

Citations 14

Authors

Maurice Schallenberg

Verena Prokosch

Solon Thanos

Affiliations

Soon will be listed here.

Abstract

Examination of the response of the retinal proteome to elevated intraocular pressure (IOP) and to the pharmacological normalization of IOP is crucial, in order to develop drugs with neuroptorective potential. We used a hereditary rat model of ocular hypertension to lower IOP with travaprost and dorzolamide applied topically on the eye surface, and examine changes of the retinal proteome. Our data demonstrate that elevated IOP causes alterations in the retinal protein profile, in particular in high-mobility-group-protein B1 (HMGB1), calmodulin, heat-shock-protein (HSP) 70 and carbonic anhydrase II expression. The changes of the retinal proteome by dorzolamide or travoprost are different and independent of the IOP lowering effect. This fact suggests that the eye drops exert a direct IOP-independent effect on retinal metabolism. Further investigations are required to elucidate the potential neuroprotective mechanisms signaled through changes of HMGB1, calmodulin, HSP70 and carbonic anhydrase II expression in glaucoma. The data may facilitate development of eye drops that exert neuroprotection through direct pharmacological effect.

Citing Articles

Proteomics-Based Identification of Retinal Protein Networks Impacted by Elevated Intraocular Pressure in the Hypertonic Saline Injection Model of Experimental Glaucoma.

Zaman K, Nguyen V, Prokai-Tatrai K, Prokai L Int J Mol Sci. 2023; 24(16).

PMID: 37628770 PMC: 10454042. DOI: 10.3390/ijms241612592.

Retinal ganglion cell-specific genetic regulation in primary open-angle glaucoma.

Daniszewski M, Senabouth A, Liang H, Han X, Lidgerwood G, Hernandez D Cell Genom. 2023; 2(6):100142.

PMID: 36778138 PMC: 9903700. DOI: 10.1016/j.xgen.2022.100142.

Mass spectrometry-based retina proteomics.

Prokai L, Zaman K, Prokai-Tatrai K Mass Spectrom Rev. 2022; 42(3):1032-1062.

PMID: 35670041 PMC: 9730434. DOI: 10.1002/mas.21786.

A Monoclonal Anti-HMGB1 Antibody Attenuates Neurodegeneration in an Experimental Animal Model of Glaucoma.

Tonner H, Hunn S, Auler N, Schmelter C, Beutgen V, von Pein H Int J Mol Sci. 2022; 23(8).

PMID: 35456925 PMC: 9028318. DOI: 10.3390/ijms23084107.

Retinal pigment epithelium degeneration caused by aggregation of PRPF31 and the role of HSP70 family of proteins.

Valdes-Sanchez L, Calado S, de la Cerda B, Aramburu A, Garcia-Delgado A, Massalini S Mol Med. 2020; 26(1):1.

PMID: 31892304 PMC: 6938640. DOI: 10.1186/s10020-019-0124-z.

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