Let-7b Regulates the Expression of the Growth Hormone Receptor Gene in Deletion-type Dwarf Chickens

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2012 Jul 12

PMID 22781587

Citations 40

Authors

Shumao Lin

Hongmei Li

Heping Mu

Wen Luo

Ying Li

Xinzheng Jia

Sibing Wang

Xiaolu Jia

Qinghua Nie

Yugu Li

Xiquan Zhang

Affiliations

Soon will be listed here.

Abstract

Background: A deletion mutation in the growth hormone receptor (GHR) gene results in the inhibition of skeletal muscle growth and fat deposition in dwarf chickens. We used microarray techniques to determine microRNA (miRNA) and mRNA expression profiles of GHR in the skeletal muscles of 14-day-old embryos as well as 7-week-old deletion-type dwarf and normal-type chickens. Our aim was to elucidate the miRNA regulation of GHR expression with respect to growth inhibition and fat deposition.

Results: At the same developmental stages, different expression profiles in skeletal muscles of dwarf and normal chickens occurred for four miRNAs (miR-1623, miR-181b, let-7b, and miR-128). At different developmental stages, there was a significant difference in the expression profiles of a greater number of miRNAs. Eleven miRNAs were up-regulated and 18 down-regulated in the 7-week-old dwarf chickens when compared with profiles in 14-day-old embryos. In 7-week-old normal chickens, seven miRNAs were up-regulated and nine down-regulated compared with those in 14-day-old embryos. In skeletal muscles, 22 genes were up-regulated and 33 down-regulated in 14-day-old embryos compared with 7-week-old dwarf chickens. Sixty-five mRNAs were up-regulated and 108 down-regulated in 14-day-old embryos as compared with 7-week-old normal chickens. Thirty-four differentially expressed miRNAs were grouped into 18 categories based on overlapping seed and target sequences. Only let-7b was found to be complementary to its target in the 3' untranslated region of GHR, and was able to inhibit its expression. Kyoto Encyclopedia of Genes and Genomes pathway analysis and quantitative polymerase chain reactions indicated there were three main signaling pathways regulating skeletal muscle growth and fat deposition of chickens. These were influenced by let-7b-regulated GHR. Suppression of the cytokine signaling 3 (SOCS3) gene was found to be involved in the signaling pathway of adipocytokines.

Conclusions: There is a critical miRNA, let-7b, involved in the regulation of GHR. SOCS3 plays a critical role in regulating skeletal muscle growth and fat deposition via let-7b-mediated GHR expression.

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References

Reinhart B, Slack F, Basson M, Pasquinelli A, Bettinger J, Rougvie A . The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature. 2000; 403(6772):901-6. DOI: 10.1038/35002607. View

Ruby J, Jan C, Player C, Axtell M, Lee W, Nusbaum C . Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans. Cell. 2006; 127(6):1193-207. DOI: 10.1016/j.cell.2006.10.040. View

Ichimura A, Ruike Y, Terasawa K, Tsujimoto G . miRNAs and regulation of cell signaling. FEBS J. 2011; 278(10):1610-8. DOI: 10.1111/j.1742-4658.2011.08087.x. View

Yang S, Xu C, Wu J, Yang G . SOCS3 inhibits insulin signaling in porcine primary adipocytes. Mol Cell Biochem. 2010; 345(1-2):45-52. DOI: 10.1007/s11010-010-0558-7. View

Yao J, Wang Y, Wang W, Wang N, Li H . Solexa sequencing analysis of chicken pre-adipocyte microRNAs. Biosci Biotechnol Biochem. 2011; 75(1):54-61. DOI: 10.1271/bbb.100530. View

Filipowicz W, Bhattacharyya S, Sonenberg N . Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?. Nat Rev Genet. 2008; 9(2):102-14. DOI: 10.1038/nrg2290. View

Arman A, Yuksel B, Coker A, Sarioz O, Temiz F, Topaloglu A . Novel growth hormone receptor gene mutation in a patient with Laron syndrome. J Pediatr Endocrinol Metab. 2010; 23(4):407-14. DOI: 10.1515/jpem.2010.064. View

Freeth J, Ayling R, Whatmore A, Towner P, Price D, Norman M . Human skin fibroblasts as a model of growth hormone (GH) action in GH receptor-positive Laron's syndrome. Endocrinology. 1997; 138(1):55-61. DOI: 10.1210/endo.138.1.4853. View

Hull K, Harvey S . Growth hormone resistance: clinical states and animal models. J Endocrinol. 1999; 163(2):165-72. DOI: 10.1677/joe.0.1630165. View

10.

Huang N, Cogburn L, Agarwal S, Marks H, Burnside J . Overexpression of a truncated growth hormone receptor in the sex-linked dwarf chicken: evidence for a splice mutation. Mol Endocrinol. 1993; 7(11):1391-8. DOI: 10.1210/mend.7.11.8114754. View

11.

Kwon C, Han Z, Olson E, Srivastava D . MicroRNA1 influences cardiac differentiation in Drosophila and regulates Notch signaling. Proc Natl Acad Sci U S A. 2005; 102(52):18986-91. PMC: 1315275. DOI: 10.1073/pnas.0509535102. View

12.

Roush S, Slack F . The let-7 family of microRNAs. Trends Cell Biol. 2008; 18(10):505-16. DOI: 10.1016/j.tcb.2008.07.007. View

13.

Ying Y, Wei H, Cao L, Lu J, Luo X . Clinical features and growth hormone receptor gene mutations of patients with Laron syndrome from a Chinese family. Zhongguo Dang Dai Er Ke Za Zhi. 2007; 9(4):335-8. View

14.

Laron Z, Pertzelan A, Mannheimer S . Genetic pituitary dwarfism with high serum concentation of growth hormone--a new inborn error of metabolism?. Isr J Med Sci. 1966; 2(2):152-5. View

15.

Touchburn S, Guillaume J, Leclercq B, Blum J . Lipid and energy metabolism in chicks affected by dwarfism (dw) and Naked-neck (Na). Poult Sci. 1980; 59(10):2189-97. DOI: 10.3382/ps.0592189. View

16.

Kanehisa M . The KEGG database. Novartis Found Symp. 2003; 247:91-101; discussion 101-3, 119-28, 244-52. View

17.

Aggrey S, Yao J, Sabour M, Lin C, Zadworny D, Hayes J . Markers within the regulatory region of the growth hormone receptor gene and their association with milk-related traits in Holsteins. J Hered. 1999; 90(1):148-51. DOI: 10.1093/jhered/90.1.148. View

18.

Pierce A, Fukada H, Dickhoff W . Metabolic hormones modulate the effect of growth hormone (GH) on insulin-like growth factor-I (IGF-I) mRNA level in primary culture of salmon hepatocytes. J Endocrinol. 2005; 184(2):341-9. DOI: 10.1677/joe.1.05892. View

19.

Glazov E, Cottee P, Barris W, Moore R, Dalrymple B, Tizard M . A microRNA catalog of the developing chicken embryo identified by a deep sequencing approach. Genome Res. 2008; 18(6):957-64. PMC: 2413163. DOI: 10.1101/gr.074740.107. View

20.

Sabio G, Das M, Mora A, Zhang Z, Jun J, Ko H . A stress signaling pathway in adipose tissue regulates hepatic insulin resistance. Science. 2008; 322(5907):1539-43. PMC: 2643026. DOI: 10.1126/science.1160794. View